| 摘要: |
| 【目的】研究OCX-32基因在淮南麻黄鸡、五华鸡、淮北麻鸡和皖南三黄鸡等4个安徽省地方鸡品种中的遗传多态性及OCX-32基因与淮南麻黄鸡蛋品质的相关性，为揭示这些群体的遗传特征提供基础数据。【方法】采用PCR-RFLP技术，对淮南麻黄鸡、五华鸡、淮北麻鸡和皖南三黄鸡4个地方鸡品种的OCX-32基因外显子4进行遗传多态性研究，并对外显子4遗传多态性与淮南麻黄鸡蛋品质的相关性进行分析。【结果】OCX-32基因外显子4存在A494C 1个单核苷酸多态性位点。该位点在安徽省4个地方鸡品种中的基因频率和基因型频率分布差异不大，C等位基因频率较高。4个地方鸡品种群体在A494C位点处于Hardy-Weinberg平衡状态，均表现为中度多态。相关分析结果表明，OCX-32基因外显子4的A494C位点基因多态性与淮南麻黄鸡的开产蛋质量、30周龄蛋质量、蛋白高度和哈氏单位显著相关（P<0.05）；CC基因型的开产蛋质量和30周龄蛋质量显著高于AA基因型，AA和CC基因型的蛋白高度显著高于AC基因，AA基因型的哈氏单位显著高于AC基因型（P<0.05）。【结论】OCX-32基因与淮南麻黄鸡蛋品质存在一定的相关性，可作为发掘淮南麻黄鸡蛋品质的分子育种标记。 |
| 关键词: OCX-32基因 淮南麻黄鸡 蛋品质 安徽省 |
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| 基金项目:国家蛋鸡产业技术体系项目（CARS-41-K19）；安徽省农业科学院院长青年创新基金项目(13B0425) |
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| Polymorphisms of exon 4 of OCX-32 gene in four indigenous chicken breeds in Anhui Province and its relationship with egg quality of Huainan partridge chicken |
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LI Jun-ying,ZHAN Kai,BI Shan-hui,et al
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| Abstract: |
| 【Objective】This study was aimed to analyze the genetic variations of OCX-32 gene of Huainan partridge chicken,Wuhua chicken,Huaibei ma chicken and Wannan sanhuang chicken in Anhui Province,and investigate the relationship between OCX-32 gene and egg quality of Huainan partridge chicken in order to reveal these populations’ genetic characteristics and provide data for marker-assisted selection of egg quality in future.【Method】The polymorphisms of exon 4 of OCX-32 gene were analyzed by PCR-RFLP technique in Huainan partridge chicken,Wuhua chicken,Huaibei ma chicken,Wannan sanhuang chicken and the effects of genotypes of exon 4 in OCX-32 gene on egg quality traits of Huainan partridge chicken were estimated.【Result】A mutation of A to G was found in the exon 4 of OCX-32 gene (A494C).The genotype and allele frequencies in the four domestic breeds varied similarly and allele C was higher.The four populations were at Hardy-Weinberg equilibrium at A494C site (P<0.05) and showed moderate polymorphism.Significant effects of A494C in the exon 4 of OCX-32 gene on egg weight at first laying,egg weight at 30th week,albumen height and Haugh unit were observed in Huainan partridge chicken population (P<0.05).The chickens with CC genotype had higher egg weight of first laying and egg weight at 30th week than that of genotype AA genotype (P<0.05).The albumen height of AA and CC genotype were higher than that of AC genotype (P<0.05),and Haugh unit of AA genotype was higher than that of AC genotype in Huainan partridge chicken (P<0.05).【Conclusion】OCX-32 gene affected egg quality and could be used as molecular breeding marker for egg quality of Huainan partridge chicken. |
| Key words: OCX-32 gene Huainan partridge chicken egg quality Anhui Province |
