| 摘要: |
| 【目的】分析鸡卵母细胞卵黄生成受体(OVR)基因5′侧翼区多态性与鸡蛋胆固醇含量的关联性。【方法】以丝羽乌骨鸡为研究对象，测定936枚丝羽乌骨鸡蛋黄和全蛋胆固醇含量，利用聚合酶链式反应 限制性片段长度多态性(PCR-RFLP)方法结合PCR产物测序技术，对丝羽乌骨鸡OVR基因5′侧翼区进行多态性检测，并分析其多态性与鸡蛋胆固醇含量的关联性。【结果】丝羽乌骨鸡蛋黄胆固醇含量为13.411 mg/g，全蛋胆固醇含量平均为456.502 mg/hg。在鸡OVR基因5′侧翼区检测到g.9511G>A和g.10428T>C 2个突变位点，其与鸡蛋蛋黄和全蛋胆固醇含量显著或极显著相关，胆固醇含量较低的基因型分别是GG和TT(P<0.05)。【结论】鸡OVR基因5′侧翼区多态性对鸡蛋胆固醇含量有一定影响。 |
| 关键词: 丝羽乌骨鸡 OVR基因5′侧翼区 SNPs 鸡蛋胆固醇含量 |
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| 基金项目:河南省科技创新人才（杰出青年）基金项目（104100510013）；河南省博士后科研项目（2011034） |
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| Association between polymorphism in the 5′ flank region of oocyte vitellogensis receptor gene and egg cholesterol content |
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ZHU Shi-kang,LÜ Shi-jie,LIU Xiang-bo,et al
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| Abstract: |
| 【Objective】This study aimed to analyze the association between the polymorphism in 5′ flank region of chicken oocyte vitellogensis receptor gene (OVR) and egg cholesterol content.【Method】Silky fowls were used as the research subjects.A total of 936 silky eggs were used to measure the cholesterol contents in yolk and the whole egg.Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and PCR products sequencing method were used to detect the polymorphisms (SNPs) in the 5′ flank region of the OVR gene,and analyze the association between SNPs and egg cholesterol content.【Result】The average cholesterol contents in yolk and the whole egg were 13.411 mg/g and 456.502 mg/hg,respectively.Two SNPs (g.9511G>A and g.10428T>C) were significantly or extremely significantly associated with the cholesterol contents in yolk and the whole egg.Genotypes GG and TT had the lowest cholesterol contents (P＜0.05).【Conclusion】OVR gene had certain effects on the egg cholesterol content. |
| Key words: silky fowls 5′ flank region of the OVR gene SNPs cholesterol content of eggs |
