| 摘要: |
| 【目的】比较獭兔和中系安哥拉兔背最长肌、皮下和肠系脂肪组织中脂肪酸的组成。【方法】采用氯仿-甲醇超声法提取獭兔和中系安哥拉兔背最长肌、皮下和肠系脂肪组织中的油脂，用碱法甲酯化法对油脂进行处理，利用气相色谱-质谱联用仪对脂肪酸进行定性和定量分析。【结果】獭兔和中系安哥拉兔的不饱和脂肪酸平均相对含量分别为59.98%和66.10%，均高于饱和脂肪酸，是脂肪酸的主体成分。在獭兔和中系安哥拉兔的背最长肌、皮下和肠系脂肪组织中，软脂酸(十六碳酸，C16:0)、油酸(9-十八碳烯酸，C18:1)和亚油酸(9，12-十八碳二烯酸，C18:2)都是脂肪酸的主要组成成分。獭兔相对含量最大的脂肪酸是软脂酸(平均相对含量为27.63%)，而中系安哥拉兔则含油酸最多(平均相对含量为32.41%)。【结论】獭兔和中系安哥拉兔脂肪酸组成具有共性，中系安哥拉兔脂肪酸种类较獭兔丰富。 |
| 关键词: 獭兔 中系安哥拉兔 脂肪酸 气相色谱 质谱联用仪 |
| DOI： |
| 分类号: |
| 基金项目:陕西省农业攻关项目（2010K01-16）；西安市农业攻关项目（NC09049-4） |
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| Comparison on fatty acid compositions in different tissues of Rex rabbit and Angora rabbit |
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CAO Wei-na,REN Zhan-jun
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| Abstract: |
| 【Objective】This study aimed to compare fatty acid composition in longissimus dorsi,subcutaneous adipose tissue,and intestinal fat tissue of Rex rabbit and Angora rabbit.【Method】Chloroform-methanol hyperacoustic method was used for lipid separation,then alkaline methyl-esterification method was used for further process,and gas chromatography and mass spectrometry technique was used to determine the fatty acid composition in longissimus dorsi,subcutaneous adipose tissue,and intestinal fat tissue of studied rabbits.【Result】Unsaturated fatty acids accounted for the majority of the averaged fatty acids in both Rex rabbit (59.98%) and Angora rabbit (66.10%).Palmitic acid (C16:0),oleic acid (C18:1) and linoleic acid (C18:2) were the main components.Content of oleic acid was the largest in Rex rabbit (27.63%),while content of palmitic acid (C16:0) was the largest in Angora rabbit (32.41%).【Conclusion】Fatty acid composition of the two rabbits was in common,and the type of fatty acids of Angora rabbit was richer than that of Rex rabbit. |
| Key words: Rex rabbit Angora rabbit fatty acids GC/MS |
