| 摘要: |
| 【目的】研究外源肉碱对鲤鱼背肌脂肪酸组成及其CPTI基因表达的影响，为阐明肉碱调控脂肪酸代谢的机理提供理论依据。【方法】在基础饲料中分别添加0，50，100，150，200和400 mg/kg L-肉碱，进行饲养试验，60 d后取鲤鱼（Cyprinus carpio）背肌样品，测定其脂肪酸组成；提取鲤鱼背肌总RNA，采用同源克隆方法扩增CPTI基因的部分序列，Real-time PCR方法测定CPTI基因mRNA表达水平的变化。【结果】外源肉碱对鲤鱼背肌脂肪酸组成有显著影响（P<0.05），随饲料中L 肉碱添加量的增加，鲤鱼背肌∑SFA和∑MUFA脂肪酸含量显著升高（P<0.05），∑PUFA、EPA、DHA、∑n-3和∑n-6脂肪酸含量显著降低（P<0.05）；同源克隆扩增获得了鲤鱼CPTI基因的部分cDNA序列（GenBank注册号为JQ361077），长度为857 bp，同源性分析表明其与斑马鱼（Danio rerio）的同源性为89%。鲤鱼饲料中L-肉碱添加量为150 mg/kg时，CPTI基因mRNA的表达丰度最高，为对照组的4倍。【结论】本试验条件下，外源肉碱添加量为150 mg/kg时，显著影响鲤鱼幼鱼背肌脂肪酸组成和提高CPTI基因mRNA表达水平。 |
| 关键词: 外源肉碱 鲤鱼 脂肪酸组成 CPT I基因表达 |
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| 基金项目:国家自然科学基金项目（30671621，31302149）；沈阳农业大学青年教师科研基金项目（20111015）；吉林省发改委产业技术研究与开发项目；东北老工业基地外贸发展项目 |
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| Effects of exogenous carnitine on fatty acid composition and CPT I gene expression in muscle of common carp（Cyprinus carpio） |
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DONG Jing,LI Lin,YU Ye-hui,et al
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| Abstract: |
| 【Objective】Effects of exogenous carnitine on fatty acid composition and CPTI gene expression in muscle of common carp (Cyprinus carpio) were studied to better clarify the regulatory mechanism of carnitine on fatty acid metabolism.【Method】Six carp groups were fed with basal diet containing L-carnitine concentrations of 0,50,100,150,200,and 400 mg/kg for sixty days,and muscle samples were extracted for detecting fatty acid composition and RNA were extracted for CPTI gene expression measurement using Real-time PCR.【Result】 Fatty acid composition was significantly affected (P<0.05) by L-carnitine.∑SFA and ∑MUFA contents of carps fed with L-carnitine were significantly (P<0.05) higher than those of the control group,while ∑PUFA,EPA,DHA,∑n-3 and ∑n-6 contents were significantly (P<0.05) lower.Partial cDNA of CPTI gene was cloned (GenBank accession number JQ361077) by homologous cloning,and the homologous alignment was analyzed.The obtained cDNA sequence was 857 bp in length,and its homology with zebra fish (Danio rerio) was 89%.150 mg/kg L-carnitine resulted in the highest mRNA expression level,which was three times higher than that of the control group.【Conclusion】Adding 150 mg/kg L-carnitine to the basal diet significantly affected the fatty acid composition and CPTI gene expression level in muscle of common carp. |
| Key words: exogenous carnitine common carp fatty acid composition CPT I gene expression |
