| 摘要: |
| 【目的】分析生态条件和种质因素对烤烟次生代谢产物含量的影响，为低危害烟叶生产提供理论依据。【方法】从贵州道真县、威宁县、盘县和天柱县采集K326、云烟87和贵烟1号3个烟草品种的C3F和B2F等级烟叶初烤样品，测定不同烟叶样本有机酸类、生物碱、多酚类、甾醇类物质等主要次生代谢产物含量，分析烤烟次生代谢产物含量的品种和地区差异性。【结果】同一地区3个品种(系)间的有机酸和生物碱含量差异不显著；道真县和盘县不同品种间烟叶多酚类物质含量、威宁县和盘县不同品种间烟叶甾醇类物质含量的差异均不显著；威宁县、天柱县不同品种间烟叶多酚类物质含量和道真县、天柱县不同品种间烟叶甾醇类物质含量的差异达显著或极显著水平。不同地区不同品种烟叶间的有机酸、生物碱、多酚和甾醇类物质含量差异明显，地区间烟叶主要次生代谢产物总含量的差别明显大于品种间。【结论】生态条件对烟叶次生代谢产物含量影响明显，种质因素对烟叶次生代谢产物含量影响较小。 |
| 关键词: 烤烟 次生代谢物质 有机酸 生物碱 多酚 甾醇 种质因素 生态条件 |
| DOI： |
| 分类号: |
| 基金项目:中国烟草总公司贵州省烟草公司重点科技计划项目(2009-09，2013-07)；中国科学院2011年度“西部之光”人才培养计划项目 |
|
| Variation of secondary metabolites contents in flue-cured tobacco leaves from different varieties and regions |
|
GENG Zhaoliang, LI Jixin, FENG Yonggang,et al
|
|
Guizhou Academy of Tabacco Sciences,et al
|
| Abstract: |
| 【Objective】This paper studied the variation of secondary metabolites contents in flue-cured tobacco leaves from different species and regions.【Method】Flue-cured tobacco leaf samples with grades of C3F and B2F of K326,Yunyan 87 and Guiyan 1 varieties were selected from Daozhen,Weining,Panxian and Tianzhu counties in Guizhou province.Their secondary metabolites contents including organic acids,alkaloids,polyphenols and sterols were measured and the differences in them were analyzed.【Result】No significant differences in contents of organic acid and alkaloid were found among tobacco varieties from same areas.Polyphenol contents in varieties from Daozhen and Panxian countries and sterol contents in varieties from Weining and Panxian countries were not significantly different as well.Nevertheless,there were significant differences or extremely significant differences in polyphenol contents between Weining and Tianzhu countries and in sterol contents between Daozhen and Tianzhu countries.Differences in contents of organic acids,alkaloids,polyphenols and sterols of different flue-cured tobacco varieties from different areas were significant.The differences in total contents of secondary metabolites between regions were greater than that between varieties.【Conclusion】Ecological conditions had great effects on the contents of secondary metabolites in tobacco leaves,while genetic factors had less effects. |
| Key words: flue-cured tobacco secondary metabolites organic acids alkaloids polyphenols sterols genetic factor ecological condition |
