| 摘要: |
| 【目的】调查陕西地区猪圆环病毒2型(Porcine circovirus type 2,PCV2)的分子流行病学变化趋势，为进一步预防和治疗猪圆环病毒病提供参考。【方法】于2010-2012年采集陕西西安、咸阳、宝鸡、榆林和延安等地区发病猪群中的141份样品。首先，采用PCR检测样品中PCV2感染情况；然后，通过基因型特异的PCR，检测PCV2阳性样品中PCV2a和PCV2b亚型的感染情况；最后，扩增、克隆和测序PCV2 ORF2基因编码序列，并通过生物信息学软件统计分析所获得基因序列的进化情况。【结果】141份样品中，117份检测为PCV2阳性，阳性率达82.9%，其中的33份病死猪样品中有32份检测为PCV2阳性。基因型特异的PCR检测结果显示，117份PCV2阳性样品中单感染PCV2b的为59份，单感染PCV2a的为11份，另外47份样品为PCV2a和PCV2b混合感染。对32份病死猪PCV2阳性样品的PCV2 ORF基因序列进行比对分析，结果表明，12份为PCV2b 1A/1B亚型，16份为PCV2b 1C亚型，4份为PCV2a亚型。对各亚型ORF2氨基酸序列的分析结果表明，氨基酸的变异位点主要存在于8-47，59-101，134-169，180-210这4个区域。【结论】陕西地区猪群PCV2感染严重，并且PCV2b-1A/1B和PCV2b-1C亚型是主要流行毒株，氨基酸变异位点主要存在于Cap蛋白潜在表位区。 |
| 关键词: 猪圆环病毒2型 PCV2a PCV2b ORF2 序列分析 流行株 |
| DOI： |
| 分类号: |
| 基金项目:西北农林科技大学基本科研业务费项目（QN2011064）；西北农林科技大学引进人才启动项目（Z111021103） |
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| Molecular epidemiological investigation on porcine circovirus type 2 in Shaanxi Province during 2010-2012 |
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SHAO Meng, LV Yahui, DU Qian
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NorthwestA&FUniversity
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| Abstract: |
| 【Objective】The study investigated molecular epidemiology of province circovirus type 2 (PCV2) to improve the prevention and clinical treatment of PCV2-infected pigs.【Method】A total of 141 samples were collected in 2010-2012 from disease pigs in different areas including Xi’an,Xianyang,Baoji,Yulin,Yan’an in Shaanxi Province,China.First,the incidence of PCV2 in these 141 samples was identified by classical PCR.Then the subgroup of PCV2 positive samples was further identified by genotype-specific PCR assay.Finally,full-length ORF2 genes of PCV2 isolates were amplified,cloned and sequenced,and the sequences were analyzed by bioinformatics software.【Result】117/141(82.9%) disease pigs were positive to PCV2,while 32/33 dead pigs were positive.Genotype-specific PCR results showed that 59 of 117 PCV2 positive samples were identified as PCV2b-single infection,11 of 117 were identified as PCV2a-single infection,and 47 of 117 were identified as mixed infection with PCV2a and PCV2b.Analysis of the PCV2 ORF2 sequences of 32 samples collected from dead pigs showed that 12/32 belonged to PCV2b-1A/1B,16/32 belonged to PCV2a-1C,and 4/32 belonged to PCV2a.Analysis of ORF2 acid amino sequences showed that most of mutation sites located at four regions (8-47,59-101,134-169 and 180-210).【Conclusion】PCV2 infection was serious in Shaanxi Province,PCV2b-1A/1B and PCV2b-1C were epidemic strains.Amino mutation sites mainly located at Cap protein potential epitomes area. |
| Key words: PCV2 PCV2a PCV2b ORF2 sequence analysis epidemic strains |
