| 摘要: |
| 【目的】探讨脱除菊花体内菊花B病毒（CVB）、黄瓜花叶病毒（CMV）及烟草花叶病毒（TMV）3种病毒的最佳方法。【方法】以菊花品种“墨菊”为材料，采用茎尖培养法、病毒唑结合茎尖培养法及热处理结合茎尖培养法对脱除CMV、CVB、TMV的效果进行研究，其中病毒唑结合茎尖培养法采用L9（34）正交试验，对茎尖长度、病毒唑质量浓度及处理时间进行筛选，热处理结合茎尖培养法中热处理采用昼夜变温并逐步升温的方式。【结果】茎尖培养法中以长度为0.4～0.5 mm茎尖的脱毒效果最佳，茎尖成活率为66.7%，CMV、CVB、TMV 3种病毒的脱毒率分别为61.9%，63.2%，55.0%；病毒唑结合茎尖培养法中以茎尖长度为0.4～0.5 mm、病毒唑质量浓度为10 mg/L、处理时间为42 d组合的脱毒效果最佳，茎尖成活率为53.3%，CMV、CVB、TMV 3种病毒的脱毒率分别为88.2%，86.7%，81.3%；热处理结合茎尖培养法中以试管苗热处理60 d后，剥取长度为0.4～0.5 mm的茎尖进行培养的脱毒效果最佳，茎尖成活率为56.7%，CMV、CVB、TMV 3种病毒的脱毒率分别为100.0%，100.0%，94.1%。【结论】3种脱除菊花体内病毒的方法中，热处理结合茎尖培养法的脱毒效果最佳。 |
| 关键词: 菊花 病毒 菊花B病毒 黄瓜花叶病毒 烟草花叶病毒 脱毒率 |
| DOI： |
| 分类号: |
| 基金项目:林业公益性行业科研专项（200904050） |
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| Effects of different methods on elimination of three viruses in Chrysanthemum morifolium |
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ZHANG Shuang, LI Qing, DAI Silan
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Beijing Forestry University
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| Abstract: |
| 【Objective】The experiment was conducted to discuss the best virus elimination method to eliminate cucumber mosaic virus (CMV),chrysanthemum virus B (CVB) and tobacco mosaic virus (TMV) in Chrysanthemum morifolium.【Method】The experiment used Chrysanthemum morifolium‘Moju’ as material.Shoot-tip culture method,shoot-tip culture with virazole treatment method and shoot-tip culture with heat treatment method were adopted to study the virus-elimination effect on CMV,CVB and TMV.An orthogonal experimental design of L9（34）was conducted to screen the best shoot-tip length,virazole concentration,processing time for the second method.For the third method, temperature increased day by day and was set differently in day time and night time.【Result】When shoot-tip length was 0.4-0.5 mm,first method obtained the best results with survival rate of 66.7% and the virus-elimination rates of CMV,CVB and TMV were 61.9%,63.2%,and 55.0%,respectively.The best conditions for method two were shoot-tip length 0.4-0.5 mm,virazole concentration 10 mg/L and processing time 42 days.The survival rate was 53.3%,and the virus-elimination rates of CMV,CVB and TMV were 88.2%,86.7%,and 81.3%,respectively.The best conditions for method three were shoot-tip length 0.4-0.5 mm and heating time 60 d.The survival rate was 56.7%,and the virus-elimination rates of CMV,CVB and TMV were 100.0%,100.0%,and 94.1%,respectively.【Conclusion】The shoot-tip culture with heat treatment method was the best to eliminate viruses in Chrysanthemum morifolium. |
| Key words: Chrysanthemum morifolium viruses CVB CMV TMV virus-elimination rate |
