| 摘要: |
| 【目的】分析无角陶赛特绵羊群体的UMP、MNS19A、CSSM18、BDKRB、MULGE6、MULGE5等 6个微卫星基因座的基因多态性，并研究这6个微卫星标记多态性对无角陶赛特绵羊早期生长发育的影响。【方法】采集新疆玛纳斯新澳畜牧有限责任公司种羊场163只无角陶赛特绵羊羔羊血液，利用微卫星分型方法分析6个微卫星标记在无角陶赛特绵羊群体中的遗传多态性，利用固定效应模型最大限度地消除或减少非遗传因素对早期生长发育的影响，然后选择基因效应显著影响的生产性状，用最小二乘方差法分析基因多态性与早期生长发育性状的相关性。【结果】微卫星标记UMP、MNS19A、CSSM18、BDKRB、MULGE6、MULGE5的等位基因数分别为3，4，4，5，5，8；平均多态信息含量为0.655，平均遗传杂合度为0.698，这6个微卫星标记在该研究群体均表现中度或者高度多态，且具有比较丰富的遗传多样性。固定效应模型分析结果显示：微卫星标记CSSM18显著影响无角陶赛特绵羊初生体质量(P＜0.05)；微卫星标记MNS19A显著影响无角陶赛特绵羊初生体质量(P＜0.05)，极显著影响2月龄胸围(P＜0.01)；微卫星标记BDKRB极显著影响无角陶赛特绵羊初生体质量、2月龄胸围、3月龄体质量(P＜0.01)，显著影响3月龄臀宽(P＜0.05)。分析比较这3个微卫星基因座不同基因型个体间的平均生产性能，结果显示微卫星标记MNS19A和BDKRB不同基因型间的平均生产性能差异显著(P＜0.05)。【结论】微卫星标记MNS19A和BDKRB可以作为无角陶赛特绵羊相应生长性状的分子筛选标记。 |
| 关键词: 无角陶赛特绵羊 微卫星标记 基因型 生长发育 |
| DOI： |
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| 基金项目:新疆维吾尔自治区国际科技合作项目(201141143) |
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| Associations of microsatellite DNA markers on chromosome 18 with early growth traits of Poll Dorset sheep |
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YANG Dan, NIU Zhigang, SHI Hongcai,et al
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College of Animal Science and Technology ,Shihezi University,et al
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| Abstract: |
| 【Objective】The aim of this study was to investigate the effects of polymorphisms of microsatellite DNA markers on chromosome 18 on early growth traits of Poll Dorset sheep.【Method】Genetic polymorphisms of 6 microsatellite markers (UMP,MNS19A,CSSM18,BDKRB,MULGE6,and MULGE5) from 163 Poll Dorset sheep were analyzed.The fixed effects model was used to eliminate or reduce the effects of non-genetic factors on early growth and development.Then,the correlation between genotype polymorphisms and early growth traits was assayed.【Result】The numbers of alleles in UMP,MNS19A,CSSM18,BDKRB,MULGE6,and MULGE5 were 3,4,4,5,5,and 8,respectively.The average heterozygosity was 0.698,and the average polymorphism information content (PIC) was 0.655.CSSM18 had significant effects on birth weight (P<0.05).MNS19A had significant effects on birth weight (P<0.05) and special significant effects on bust at 2-month age (P<0.01).BDKRB had special significant effects on birth weight,bust at 2-month age,body weight at 3-month age (P<0.01) and significant effects on hip width at 3-month age (P<0.05).BDKRB and MNS19A had significant effects on early growth traits.【Conclusion】Microsatellite markers MNS19A and BDKRB can be used as molecular selection markers for corresponding growth traits. |
| Key words: Poll Dorset sheep microsatellite marker genotype growth traits |
