| 摘要: |
| 【目的】研究NaCl胁迫对变异紫叶三叶草种子萌发和保护酶活性的影响，以揭示其耐盐特性和生理机制。【方法】以变异紫叶三叶草、白三叶草（Trifolium repens L.）和红三叶草（Trifolium pratense L.）为试验材料，以蒸馏水处理为对照，在不同质量分数NaCl溶液（0.2%，0.4%，0.6%，0.8%，1.0%和1.2%）处理下，测定三叶草种子萌发生长指标及3种保护酶（SOD、POD和CAT）活性的变化规律。【结果】NaCl胁迫对变异紫叶三叶草的种子萌发和幼苗生长均有一定的抑制作用。其中，总萌发率、发芽指数、发芽值、胚根长度、胚根生长速率和活力指数等均随NaCl胁迫程度的加重不断降低，抑制程度总体高于同一处理水平下的白三叶草和红三叶草。变异紫叶三叶草的SOD和POD活性随NaCl胁迫程度的加重不断减小，而CAT活性先降低后升高；在同一NaCl处理水平下，SOD和POD活性的大小依次为红三叶草＞白三叶草＞变异紫叶三叶草，而CAT活性大小依次为红三叶草＞变异紫叶三叶草＞白三叶草。【结论】变异紫叶三叶草的耐盐性较白三叶草和红三叶草弱，但其紫叶的观赏价值较高。 |
| 关键词: 变异紫叶三叶草 NaCl胁迫 种子萌发 保护酶活性 |
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| 基金项目:西安市园林观赏花卉示范园建设子项目（NC10031（2）） |
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| Effects of NaCl stress on seed germination and activities of protective enzymes in purple clover variants |
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WU Nan, WANG Fei, TIAN Zhiguo,et al
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Northwest A&F University,et al
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| Abstract: |
| 【Objective】The effects of NaCl stress on seed germination and activities of protective enzymes in purple clover variants were studied in order to reveal the salt tolerance and physiological response mechanism.【Method】Taking purple clover variants,Trifolium repens L.and Trifolium pratense L.as test materials,the seed germination rate,germination index,germination value,vigor index,and growth of radicels coupled with the activities of three protective enzymes (SOD,POD and CAT) were investigated under the treatment of NaCl solutions with gradient concentrations of 0 (distilled water as the control),0.2%,0.4%,0.6%,0.8%,1.0% and 1.2%.【Result】Results showed that the germination of seeds and seedlings of purple clover variants were inhibited by NaCl stress.With the increase of NaCl concentration,the seed germination rate,growth of radicels,germination value,germination index,root length and vigor index decreased gradually.The suppressive effect with same treatment on purple clover was more significant than T.repens L.and T.pratense L.For purple clover variants,activities of SOD and POD decreased continuously,while the activity of CAT increased gradually with the aggravation of NaCl stress.Under the same treatment,activities of the three enzymes in purple clover variants were lower than that in T.repens L.and T.pratense L.【Conclusion】Compared with T.repens L.and T.pratense L.,the salt tolerance of purple clover variants was relatively weaker,but the ornamental character was more valuable. |
| Key words: purple clover from variants NaCl stress seed germination activity of protective enzymes |
