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甘草提取物对卡拉库尔绵羊血液抗氧化性能的影响
刘俊峰1,2, 郭雪峰, 刘永宏
1.新疆生产建设兵团塔里木畜牧科技重点实验室;2.塔里木大学 动物科学学院
摘要:
【目的】研究甘草提取物对卡拉库尔绵羊血液抗氧化性能的影响。【方法】选取健康且体质量相近的6只卡拉库尔绵羊,平均体质量为18.65 kg/只,随机分为试验组和对照组,分别饲喂添加甘草提取物的基础日粮(甘草提取物添加量150 mg/kg)及基础日粮,在正试期的第15,30和45天时静脉采血,分离血清和血浆,检测血液中总抗氧化能力(T-AOC)、羟自由基(OH·)、谷胱甘肽过氧化物酶(GSH-PX)、丙二醛(MDA)和总超氧化物歧化酶(T-SOD)的活性。【结果】试验组卡拉库尔绵羊的T-AOC、GSH-PX、T-SOD活性显著高于对照组(P<0.05),而OH·试验组显著低于对照组(P<0.05),试验组的MDA含量与对照组相比差异不显著(P>0.05)。【结论】甘草提取物可提高卡拉库尔绵羊的抗氧化能力。
关键词:  甘草提取物  总抗氧化能力  羟自由基  谷胱甘肽过氧化物酶  丙二醛  总超氧化物歧化酶  卡拉库尔绵羊
DOI:
分类号:
基金项目:新疆生产建设兵团塔里木畜牧科技重点实验室开放课题(HS201106);兵团博士资金专项(2009JC08)
Effects of licorice extract on blood antioxidant properties of Karakul sheep
LIU Junfeng1,2, GUO Xuefeng, LIU Yonghong
1.Key Laboratory of Animal Science;2.Tarim University
Abstract:
【Objective】Blood antioxidant properties of Karakul sheep fed with licorice extract were studied.【Method】6 healthy Karakul sheep with similar weights (averaged weight was 18.65 kg) were randomly divided into experimental group and control group,3 repeats for each group.Experimental group were fed with diets supplemented with licorice extract.Blood samples were collected from neck vein of each sheep at day 15,30 and 45,respectively.Serum and plasma were separated before the blood total antioxidant capacity (T-AOC),hydroxyl free radical (OH·),glutathione peroxidases (GSH-PX),malondialdehyde(MDA)and total super oxide dismutase(T-SOD)vitality were detected.【Result】The results showed that T-AOC,GSH-PX,and T-SOD of the experimental group were significantly higher (P<0.05) than that of the control group,while the OH· of the experimental group were significantly lower (P<0.05) than that of the control group.There was no significant difference of MDA between experimental group and control group (P>0.05).【Conclusion】Licorice extract used in this study could improve the antioxidant capacity of Karakul sheep.
Key words:  licorice extract  total antioxidant capacity  hydroxyl radical  glutathione peroxides  millennial dehyde  superoxide dismutase  Karakul sheep

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