| 摘要: |
| 【目的】研究了茶枯饼乙醇抽提物对爪哇根结线虫（Meloidogyne javanica）的毒杀活性，以及茶枯饼粉末拌土处理对空心菜和落葵爪哇根结线虫病的盆栽防治效果，为线虫病的生物防治提供理论依据。【方法】以茶枯饼为材料，采用药液直接浸泡法和粉末拌土盆栽法，分别研究了茶枯饼乙醇抽提物对爪哇根结线虫2龄幼虫的毒杀活性、对卵的孵化抑制性，以及茶枯饼粉末拌土处理对空心菜和落葵爪哇根结线虫病的防效。【结果】茶枯饼乙醇抽提物对爪哇根结线虫2龄幼虫有较强的毒杀活性，以50，100，150 mg/mL茶枯饼乙醇抽提物处理72 h，经清水复苏24 h后，校正死亡率分别为39.94%，58.80%，70.68%；对爪哇根结线虫卵的孵化表现出较强抑制活性，以50，100，150 mg/mL茶枯饼乙醇抽提物处理5 d后，抑制孵化率均达到58%以上。茶枯饼粉末拌土处理对空心菜（9 g/L）及落葵（11 g/L）爪哇根结线虫均有较好的防治效果，茶枯饼粉末拌土能显著降低根结数及雌虫产卵量，并使植株地上部鲜质量显著增加。【结论】茶枯饼中存在杀线虫成分，可作为潜在的防治根结线虫病资源用于线虫病的防治。 |
| 关键词: 茶枯饼 爪哇根结线虫 杀线活性 |
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| 基金项目:公益性行业（农业）科研专项经费项目（nyhyzx07-050-4）；广东省农业攻关项目（2007B020709008） |
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| Nematicidal activity of ethanol extracts from tea cakes against Meloidogyne javanica |
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LI Lili, LIAO Jinling, WEN Yanhua
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South China Agricultural University
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| Abstract: |
| 【Objective】In this paper,ethanol extracts from tea cakes were assessed for their nematicidal activity against Meloidogyne javanica,and pot trial was also conducted to adjust the control effects of tea cake powder mixed with the soil treatment against diseases caused by M.javanica.【Method】Tea cakes were the research medium.The liquid immersion method and potting method with powder mixed with the soil were used to study the nematicidal activity of ethanol extracts from tea cakes against Meloidogyne javanica,the rejection capability to egg hatchings,and the control effects on water spinach and basella alba.【Result】Thanol extracts from tea cakes had strong nematicidal activity against the second stage juveniles.The adjusted mortality of M.javanica juveniles,after being treated by ethanol extracts with concentrations of 50,100 ,and 150 mg/mL for 72 h and being put in water for 24 h,were 39.94%,58.80%,and 70.68%,respectively,Ethanol extracts from tea cakes also had strong inhibitory activity against the egg hatching of M.javanica.The inhibitory rates to egg hatching reached 58% after being treated for 5 d with ethanol extracts with concentrations of 50,100,and 150 mg/mL.Pot trails of water spinach and basella alba showed that the root-knot disease caused by M.javanica could be effectively controlled with 1 L soil mixed with 11 g tea cake powder.The galls and eggs were reduced and fresh weight of shoots increased significantly.【Conclusion】Tea cakes contain nematicidal components and could be used to control root-knot diseases potentially. |
| Key words: tea cakes Meloidogyne javanica nematicidal activity |
