| 摘要: |
| 链霉菌噬菌体φC31整合酶是一种位点特异整合酶（Site specific recombinase,SSR），其能够精确并单向的促进attP位点与attB位点的重组，是继Cre/Loxp、FLP之后又一个倍受关注的位点特异整合酶，该整合酶已经成为基因治疗、转基因研究等领域的常用技术手段。现对φC31位点特异性整合酶的作用机制、整合位点及其在转基因和基因治疗中的应用进行了综述，并对其发展前景进行了展望。 |
| 关键词: φC31整合酶 位点特异性整合 转基因 基因治疗 |
| DOI： |
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| 基金项目:国家转基因重大专项（2011ZX08007-002）；山东省科技攻关项目（2009GG20002032）；济南市高校院所自主创新计划项目（201004027）；国家自然科学基金项目（31072160） |
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| Mechanism and application of the site-specific recombinase φC31 |
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SHANGGUAN Tao1, ZHU Qijun2, SUN Tao,et al1
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1.Shandong Academy of Agricultural Sciences;2.Animal Husbandry and Veterinary Bureau,Taian,Shandong
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| Abstract: |
| Streptomyces bacteriophage φC31 is a site specific integrase,which can catalyze precise unidirectional recombination between attP and attB recognition sites.It is another focus of site specific recombinase after Cre/Loxp and FLP.The integrase has become a common technique in the field of gene therapy and genetical modification.This paper mainly describes the mechanism,the integrate sites and the application and development prospects in transgenic modification and gene therapy of site specific recombinase φC31. |
| Key words: φC31 integrase site-specific recombinase transgenic gene therapy |
