| 摘要: |
| 【目的】研究自拟中药方剂对鸡传染性支气管炎（IB）的防治效果。【方法】首先选SPF鸡胚测定鸡传染性支气管炎病毒标准株M41型（IBV-M41）的ELD50以确定攻毒剂量，然后将400只19日龄无IB抗体的健康雏鸡随机分为8组，每组50只，分别设为预防高(2.0 mL/次)、中(1.0 mL/次)、低(0.5 mL/次)组和治疗高(4.0 mL/次)、中(2.0 mL/次)、低(1.0 mL/次)组及阳性对照组（攻毒后不给药）、阴性对照组（不攻毒、不给药），19日龄时预防组开始给予供试中药水提液，连用5 d。21日龄时，除阴性对照组外其余各组均以100ELD50剂量的IBV-M41人工感染雏鸡，待症状出现24 h后，治疗组开始给予供试中药方剂，连用5 d，期间进行临床观察，并测定不同处理组不同日龄患病雏鸡血清中的IFN-γ和TNF-β质量浓度。【结果】临床试验表明，预防组随药物剂量的增大，鸡群的发病率逐渐降低；治疗组随药物剂量的增大，鸡群的治愈率逐渐提高。对人工感染雏鸡血清中细胞因子含量变化的测定表明，预防组21日龄雏鸡未攻毒时，不同剂量组的IFN-γ和TNF-β质量浓度与阴性对照组相比均差异显著，在用药后的25，28日龄，感染IBV-M41雏鸡血清中的IFN-γ质量浓度显著高于阴性、阳性对照组，但组间差异不显著；治疗组用药2 d后，用药组之间IFN-γ质量浓度无显著性差异，但TNF-β质量浓度差异显著；28，30日龄时，IFN-γ、TNF-β 2种细胞因子的质量浓度均显著升高，并且与阴性、阳性对照组差异显著。【结论】供试中药方剂可以持续诱生IFN-γ和TNF-β，发挥了调节免疫机能的作用，从而对机体产生免疫保护，以达到对鸡传染性支气管炎的防治效果。 |
| 关键词: 鸡传染性支气管炎 中药方剂 IFN-γ TNF-β |
| DOI： |
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| 基金项目:中央级公益性科研院所基本科研业务费专项(BRF090403)；“十一五”国家科技支撑计划项目（2008BADB4B01-2） |
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| Prevention and treatment experimentation of self-made Chinese herbal compound in chicken IBV-M41 challenged |
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WANG Meng1, YAN Zuoting2, YE Dehe1
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1.College of Veterinary Medicine ,Gansu Agricultural University;2.Lanzhou Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences
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| Abstract: |
| 【Objective】The study was carried out to investigate the effect of the Chinese herbal medicine on cytokines in serum during prevention and treatment of fowl infectious bronchitis in chicken.【Method】Four hundred 19-day-old chicken with IB antibody free were divided into eight groups as follows:3 groups treated with different concentrations:high(2.0 mL/time),middle (1.0 mL/time)and low(0.5 mL/time) dose of the herbal solution for preventive trial,another 3 groups treated with different concentrations:high(4.0 mL/time),middle(2.0 mL/time) and low dose(1.0 mL/time) of the herbal solution for treatment trial,positive control group and negative control group,50 in each group.The preventive groups were treated with herbal solution on day 19 for 5 days.All the groups were challenged by IBV M41 on day 21 except the negative control group.The treatment groups underwent herbal solution for 5d after the clinic symptom coming.The clinic observed changes were recorded during the whole experimentation.The activities of interferon-γ(IFN-γ) and tumor necrosis factor-β in serum in chicken from all groups were measured and analyzed at different time.【Result】In preventive trail,the contents of IFN-γ and TNF-β in serum from chicken treated with 3 different concentrations of the herbal solution elevated significantly compared with positive control and negative control groups on Day 21 before challenged by IBV M41,and on Day 25 and Day 28 as well.In treatment trial,the concentration of IFN-γ in serum from chicken treated with 3 different concentrations of the herbal solution for 2 days displayed no significant difference,while concentration of TNF-β showed significant difference;the concentration of the two cytokines from chicken treated with 3 different concentrations of the herbal solution augmented significantly on Day 28 and Day 30 compared with those of the positive and negative control groups.【Conclusion】The self made Chinese herbal can impose a positive action on prevention and treatment of fowl infectious bronchitis in chicken by inducing INF-γ and TNF-β persistently so that it adjusts the immunity function to achieve the aim of antiviral action. |
| Key words: infectious bronchitis self-made Chinese herbal compond interferon-γ tumor necrosis factor-β |
