| 摘要: |
| 【目的】明确百合根系分泌物不同组分的化感作用，为揭示百合连作障碍产生的机理奠定理论基础。【方法】通过水培法收集百合根系分泌物，分别用石油醚、乙醚、乙酸乙酯、氯仿萃取及甲醇层析2种方法分离百合根系分泌物，获得不同组分；采用生物测试法，以萝卜为受体检测百合根系分泌物不同组分的化感作用。【结果】各组分对萝卜发芽率、苗高和苗鲜质量均表现出抑制效应，而对根鲜质量均表现出促进效应；甲醇、氯仿和石油醚组分对萝卜发芽指数、发芽势、根长表现出抑制效应，而乙酸乙酯和乙醚组分则表现出促进效应；从化感效应指数来看，甲醇、氯仿、石油醚和乙醚组分对萝卜种子萌发和幼苗生长表现出抑制作用，而乙酸乙酯组分则表现出促进作用。各组分化感效应大小的顺序为甲醇组分>氯仿组分>石油醚组分>乙酸乙酯组分>乙醚组分。各组分均导致萝卜幼苗丙二醛含量增加，吲哚乙酸氧化酶活性升高；各组分对萝卜幼苗超氧化物歧化酶和过氧化物酶活性影响不显著。【结论】百合根系分泌物的不同组分均具有化感效应，其中以甲醇和氯仿组分的化感效应较强。 |
| 关键词: 百合 根系分泌物 组分分离 化感作用 萝卜 |
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| 基金项目:国家“十一五”科技支撑计划项目(2006BAD07B02-01) |
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| Allelopathy of different fractions of lily root exudates |
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| Abstract: |
| 【Objective】The experiment was done to investigate the allelopathy of different components of lily root exudates.【Method】Lily root exudates were collected with the method of hydroponic cultivation.Petroleum ether,ethyl ether,ethyl acetate,chloroform and methanol were used to isolate lily root exudates by extracting or column chromatography,which was tested by bioassay with radish as the receiver crop.【Result】All fractions showed inhibitory effect on germination rate,shoot length and shoot fresh mass of radish,but promotion effect on root fresh mass.The fractions of methanol,chloroform,petroleum ether showed inhibitory effect on germination index,germination potentiality and root length,but the fractions of ethyl acetate and ethyl ether showed promotion.On the allelopathic index the fractions of methanol,chloroform,petroleum ether and ethyl ether showed inhibitory effect on seed germination and seedling growth of radish,but the ethyl acetate fraction showed promotion effect.At the same time,the allelopathy order of different fractions was methanol,chloroform,petroleum ether,ethyl acetate and ethyl ether.All fractions led to the increase of MDA content and the activity of IAA oxidase,then the change of protective enzymes SOD and POD activities did not show obvious tendency.【Conclusion】All fractions of lily root exudates have allelopathy,however the methanol fraction and chloroform fraction have stronger effect. |
| Key words: lily root exudates fractions isolation allelopathy radish |
