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黄瓜耐热性芽苗期鉴定指标筛选及预测方程的建立
程智慧, 聂文娟, 孟焕文,等
西北农林科技大学 园艺学院
摘要:
【目的】研究高温胁迫下黄瓜芽苗期的主要生物学性状,筛选芽苗期黄瓜耐热鉴定的形态指标。【方法】以25 ℃为对照(CK)温度,以4个已知耐热性的黄瓜品系种子为试材,在不同高温(35,38,42 ℃)下发芽6 d后,测定芽苗形态指标,并进行主成分分析和回归分析。【结果】38 ℃高温发芽条件下,黄瓜幼苗的全鲜质量(X1)、去子叶鲜质量(X2)、根鲜质量(X3)、下胚轴长(X4)、活力指数(X5)与黄瓜品系耐热性的相关性比35和42 ℃下更密切,可作为耐热种质筛选的指标;主成分分析表明,第一、二主成分对耐热性评价的贡献率分别为89.03%和9.86%;通过回归分析建立了黄瓜耐热性预测方程。【结论】在38 ℃高温条件下发芽后6 d测定的X2X5,可作为黄瓜耐热性芽苗期鉴定的简易指标,利用回归方程Y=-0.347 3+0.245 0X2+0.073 5X5,可以预测黄瓜品种资源的耐热性。
关键词:  黄瓜  耐热性  芽苗期鉴定  形态指标  预测方程
DOI:
分类号:
基金项目:陕西省西安市科技计划项目农业应用技术研究(NC09041-2);国家“十一五”科技支撑计划项目(2006BAD01A7 5-05);西北农林科技大学唐仲英育种基金项目
Screening of identification indexes at seed germination stage and establishment of the forecast equation for cucumber hot tolerance
CHENG Zhihui, NIE Wenjuan, MENG Huanwen,et al
College of Horticulture ,Northwest A&F University
Abstract:
【Objective】The biological characteristics at germination and sprouting stage were studied to screen hot tolerance identification morphologic indexes for cucumber.【Method】Taking the 4 hot tolerance known straits of cucumber as the test material and 25 ℃ as the temperature control,the main morphological indexes of cucumber were investigated under different high temperature (35 ℃,38 ℃,42 ℃) conditions 6 days after germination.The principal component analysis and regression analysis were made according to these indexes.【Result】The whole plant fresh weight (X1),fresh weight of plant without cotyledon (X2),root fresh weight (X3),hypocotyl length (X4) and vigor index (X5) showed significant correlations with hot tolerance,which were suggested to be the reliable screening indexes to identify hot tolerance variety.The first and second principal component contributed 89.03% and 9.86% to the hot tolerance of cucumber,respectively.A regressive equitation was established to access cucumber hot tolerance.【Conclusion】The indexes X2 and X5 of cucumber germinated at 38 ℃ for 6 days are thought to be the simple indexes and the regressive equitation Y=-0.347 3+0.245 0X2+0.073 5X5 is selected to forecast the hot tolerance of cucumber germplasm at germination and sprouting stage.
Key words:  cucumber  hot tolerance  identification at germination stage  morphological index  forecast equation

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