| 摘要: |
| 【目的】探讨多羟基双萘醛提取物（WCT）对猕猴桃溃疡病菌（Pseudomonas syingae pv.actinidiae）的抑制作用。【方法】采用平板抑菌圈法和猕猴桃苗盆栽接菌的方法，测定WCT对猕猴桃溃疡病菌生长的抑制作用，并在7年树龄的猕猴桃树上进行田间药效试验，分析WCT对猕猴桃苗各处理的多酚氧化酶（PPO）与过氧化氢酶（POD）活性及病程相关蛋白的诱导情况。【结果】WCT对猕猴桃溃疡病菌的抑制中浓度（EC50）为11.5 mg/mL；猕猴桃苗接种WCT和病原菌后，其PPO和POD活性均保持在较高的水平，其中WCT预防处理的PPO和POD活性均较其他处理高；SDS-PAGE电泳分析结果显示，WCT处理和接种猕猴桃溃疡病原菌均可诱导产生分子质量为35 ku的蛋白。【结论】WCT对猕猴桃溃疡病菌的生长有显著的抑制作用，通过在猕猴桃树上涂药可诱导寄主产生抗病性，进而减轻病害的发生。 |
| 关键词: 多羟基双萘醛 猕猴桃溃疡病菌 多酚氧化酶 过氧化氢酶 |
| DOI： |
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| 基金项目:公益性行业科研专项“生物源农药创制与技术集成及产业化开发”（200903052）；陕西省农业攻关项目“陕西主要果树病害发生规律及控制技术研究”（2010K01-11）；陕西省农业创新项目“猕猴桃细菌性溃疡病发生规律和防治研究”（2010NKC-08）；高等学校学科创新引智计划项目（B07049） |
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| Antimicrobial Inhibition of polyhydroxy dinaphthaldehyde extracts (WCT) on Pseudomonas syringae pv.actinidiae in kiwifruit |
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WEI Haijuan, LIU Ping, YANG Yan,et al
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College of Plant Protection, Shaanxi Key Laboratory of Molecular Biology for Agriculture,Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education,Northwest A&F University
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| Abstract: |
| 【Objective】The research was conducted to explore the antibacterial activity of WCT extracts of polyhydroxy dinaphthaldehyde against Pseudomonas syingae pv.actinidiae.【Method】With inhibition zone method and and kiwifruit seedling inoculated with P.syingae pv.actinidiae method to detect the inhibition effect of WCT on the growth of P.syingae pv.actinidia,a field experiment was conducted on 7-year-old kiwi trees.Polyphenol oxidase (PPO),peroxidase(POD)and pathogenesis related protein activities induced by inoculation with WCT and P.syingae pv.actinidiae on kiwifruit were studied.【Result】The EC50 value of WCT for P.syingae pv.actinidiae was 11.5 mg/mL.The activities of PPO and POD in kiwifruit increased siginificantly when inoculated with WCT and P.syingae pv.actinidiae and maintained at a high level throughout the experiment,in which WCT prevention activity of PPO and POD was higher than that of other treatments.Both WCT and P.syingae pv.actinidiae inoculation could increase the expression of a 35 ku protein in kiwifruit leaves.【Conclusion】WCT had a significant inhibition on P.syingae pv.actinidiae in petri dishes and field,and WCT induced the host producing resistance,hence reducing disease incidence. |
| Key words: polyhydroxy dinaphthaldehyde kiwifruit bacterial canker polyphenol oxidase peroxidase |
