| 摘要: |
| 【目的】通过对自咬症水貂神经系统病理组织学变化的观察，探讨水貂自咬症与神经系统病变的关系，为水貂自咬症发病机制的研究提供参考。【方法】采集20只临床健康水貂和20只自咬症水貂的大脑、小脑、延脑和脊髓等神经系统组织，用体积分数4%多聚甲醛固定后经选样、脱水、透明、石蜡包埋、切片和HE染色后，在200～400倍光学显微镜下观察，并对比病理变化。【结果】与临床健康水貂相比，自咬症水貂大脑中普遍存在神经细胞肿胀、溶解以及神经细胞卫星现象；部分神经锥体细胞固缩变形，细胞膜周边出现空泡化，神经纤维溶解消失，神经细胞核固缩浓染；部分自咬症水貂大脑的髓质出现空泡化，神经胶质细胞膜内侧浓染；小脑中的浦肯野氏细胞普遍存在肿胀、融解以及神经髓鞘溶解现象。延脑神经细胞肿胀，大胶质细胞膜溶解消失，神经椎体细胞周边出现严重的卫星现象； 脊髓神经细胞明显肿胀，细胞膜消失，神经细胞核固化萎缩。【结论】水貂自咬症与神经细胞膜结构损伤、神经传导细胞以及神经髓鞘的病变有关。 |
| 关键词: 水貂 自咬症 神经系统 病理变化 |
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| 基金项目:西北农林科技大学青年（专项）基金项目（08080261） |
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| Histopathological study on nervous system in self-injurious mink |
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| Abstract: |
| 【Objective】This study aimed to investigate nervous system pathological changes in mink with self injurious behavior and to research the relationship between nervous system pathological changes and this abnormal behavior in order to provide reference for pathological mechanism research of self-injurious behavior.【Method】The cerebrum,cerebellum,medulla oblongata and medulla marrow from each of 20 healthy minks and 20 self-injurious minks were collected in current study.All samples were fixed in 4% paraformaldehyde,followed by dewatering,hyalining,paraffin embedding,slicing and hematoxylin eosin staining.All sections were observed at 200-400 times light microscope.【Result】Compared with clinical healthy minks,histopathological changes were found in nervous system of minks with self-injurious behavior.Swelling,lysing and microsatellite phenomenon of nerve cells were observed in the cerebrum.Many nuclei in nerve cells became pyknotic (shrunken and dark).Vacuolizations were observed in cerebrum of some minks with self injurious behavior.Intracellular membrane of neuroglia cell was deeply stained.Swollen and lysed Purkinje cells were investigated in cerebellum,and some of the myelin sheaths were lysed in this region.Neurocyte swelling,membrane ablation of macroglia and serious microsatellite phenomenon were observed in medulla oblongata of SIB mink.Nerve cells pyknotic and membrane lysed were also found in spinal marrow.【Conclusion】Self-injurious behavior of minks has correlation with pathological changes of membrane of nerve cell and nerve myelin sheath. |
| Key words: mink self-injurious behavior nervous system pathological change |
