| 摘要: |
| 【目的】探讨菌株D-5在不同条件下对杜仲种皮固体发酵时羧甲基纤维素（CMC）酶、果胶酶活力及纤维类物质去除率的变化规律，筛选利用微生物固体发酵提取杜仲胶的发酵工艺参数。【方法】以曲霉D-5为发酵菌株，研究发酵基质不同的含水率、初始pH、起爆剂用量、发酵温度对CMC酶、果胶酶活力及纤维类物质去除率的影响。【结果】发酵基质的含水率、初始pH、起爆剂用量、发酵温度对菌株D-5的酶活力及纤维类物质的去除率有显著的影响。菌株D-5发酵的适宜条件为：基质含水率为60%、初始pH 6.0、发酵温度30 ℃、起爆剂用量1.5%，在此条件下发酵6 d CMC酶及果胶酶的活力最高，发酵18 d纤维类物质去除率可达86.43%。【结论】利用微生物发酵去除纤维类物质是杜仲胶提取的重要途径之一，菌株D-5在杜仲胶提取中有一定的应用价值。 |
| 关键词: 杜仲胶 固体发酵 纤维素酶 果胶酶 纤维类物质去除率 |
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| 基金项目:中日(NEDO)合作项目“利用工业植物原料杜仲开发可持续发展的杜仲胶生产技术”(K332020908);陕西省日元贷款项目(K332020023) |
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| Screening parameters for the extraction of Eucommia rubber (EU rubber) by microbial solid fermentation |
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| Abstract: |
| 【Objective】The study aimed to investigate changes of carboxymethyl cellulase (CMC) and pectinase activity and the elimination rate of cellulosic materials caused by microbial solid fermentation (Aspergillus D-5) on Eucommia pericarp under different conditions to screen parameters for the extraction of EU-rubber by microbial solid fermentation.【Method】Aspergillus D-5 was used to investigate the effect of different moisture contents of fermentation substrate,initial pH,dosage of ignition agent and culture temperature on CMC and pectinase activity.【Result】The results showed that the moisture content of fermentation substrate,initial pH,dosage of ignition agent and culture temperature had significant effect on the enzyme activity of D-5 strain and the elimination rate of cellulosic materials.The optimal conditions for fermentation by D-5 strain was determined:moisture content of fermentation substrate was 60%,initial pH 6.0,culture temperature 30 ℃,dosage of ignition agent 1.5%.Under these conditions,the CMC and pectinase activity was the highest after 6 days of fermentation and the elimination rate of cellulosic materials was 86.43% after 18 days of fermentation.【Conclusion】Removing cellulosic materials by microbial fermentation material is one of the most important ways to extract EU-rubber.D-5 strain in the extraction of EU rubber has certain application value. |
| Key words: Eucommia rubber solid state fermentation cellulase pectinase elimination rate of fiber materials |
