| 摘要: |
| 【目的】 研究新型鸭呼肠孤病毒（NDRV）的致病性。【方法】 以NDRV分离株NP01、NP03、NPM为供试病毒，分析其对禽胚（番鸭胚、鸡胚）、部分禽类（雏番鸭、雏半番鸭、雏鸡、雏鹅）及细胞的致病性。【结果】 NDRV分离株经尿囊腔接种能100%致死12日龄番鸭胚和9日龄鸡胚。2日龄雏番鸭、雏半番鸭经腿肌、口鼻、爪垫等途径人工感染NDRV分离株后，出现了与自然病例相同的病症，NDRV分离株对雏番鸭和雏半番鸭的致死率分别为20%～53%和13%～33%，同时能从病死鸭肝脾中回收到该病毒，耐过鸭大多成为僵鸭；15日龄雏鸡和2日龄雏鹅人工感染NDRV分离株后观察20 d，均未表现出NDRV致病的临床症状。NDRV分离株具有较广的细胞亲嗜性，能在MDEF、CEF、AD293 、Marc145、Vero、ST、MDCK等细胞中增殖并产生细胞病变，病变细胞呈现巨融合状；但NDRV分离株在PK细胞中盲传3代均未出现病变。【结论】 NDRV在致病特性方面明显不同于禽呼肠孤病毒和番鸭呼肠孤病毒。 |
| 关键词: 新型鸭呼肠孤病毒 致病性 禽胚 禽类 细胞病变 |
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| 基金项目:福建省农业科学院动物传染病防控创新团队项目（STIF-Y02）；福建省公益类项目（2010R1101003-5 ） |
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| The study on the pathogenicity of new type duck reovirus |
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| Abstract: |
| 【Objiective】 The experiment was conducted to study the pathogenicities of new type duck reovirus (NDRV).【Method】 The pathogenicities of NDRV strains (NP01,NP03,NPM) were investigated in some kinds of fowls, avian embryos and cells.【Result】 These NDRV strains could cause muscovy ducklings and semi muscovy ducklings to disease and die,the mortalities of Muscovy duckling and semi muscovy ducklings were 20%-53% and 13%-30% respectively.These dead ducklings showed characteristic hemorrhagic necrotic hepatitis,the same with the clinically dead birds and the challenged virus could be reisolated from artificial infection.The survival ducks infected experimentally with isolates were markedly stunted in growth.These NDRV strains had no obvious pathogenicity to 15 day old chicken or 2 day old goose.Muscovy duck embryos and chick embryos inoculated with NDRV died completely and had the hemorrhage and necrosis in embryo livers.NDRV induced syncytium formation in MDEF,CEF,AD293,Marc145,Vero,ST and MDCK cells monolayers and no cytopathic effect（CPE） was observed in PK cell monolayer.【Result】 NDRV's pathogenicity differed from that of ARV and MDRV. |
| Key words: new-type duck reovirus pathogenicity avian embryo poultry cytopathic effect (CPE) |
