| 摘要: |
| 【目的】 研究神经肽Y(Neuropeptide Y,NPY) 免疫反应阳性物质在大鼠动情周期子宫中的分布情况及其变化规律。【方法】 采用阴道涂片法鉴定发情周期，选取情期正常的20 只雌性大鼠，均分为动情前期、动情期、动情后期和动情间期4组，处死各组大鼠，取子宫制作石蜡切片，经免疫组织化学SP 法染色后，观察 NPY 在大鼠动情周期子宫中的分布及变化规律。【结果】 在内膜层，大多数NPY 免疫阳性产物位于细胞中，小部分位于点状和细丝状纤维中。在肌层，NPY 免疫阳性产物主要位于NPY纤维中。NPY 免疫阳性产物相对表达量的t检验结果表明，子宫内膜NPY 在动情期和动情间期的表达较动情前期显著减少（P＜0.05），较动情后期极显著减少（P＜0.01）；子宫肌层NPY在动情后期的表达较动情期显著回升（P＜0.05），在动情间期的表达较动情后期极显著减少（P＜0.01）。【结论】 NPY 在不同生理时期大鼠子宫中的表达分布存在一定规律性，可能与其生理调节功能有关。 |
| 关键词: 神经肽Y 免疫组织化学SP法 动情周期 大鼠 子宫 |
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| 基金项目:陕西省自然科学基金项目(SJ08C105)；西北农林科技大学科研专项(06ZR003) |
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| Distribution of Neuropeptide Y in uterus of rats during estrus cycle |
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| Abstract: |
| 【Objective】 The research was done to study the distribution and expression variation of Neuropeptide Y(NPY) in uterus of the rats during estrus cycle.【Method】 Twenty mature female rats at normal estrus cycle were evenly divided into 4 groups:preoestrus group,estrum group,metoestrus and diestrus group.Estrus cycle was judged by vaginal smear,the expression of NPY in uterus of the rats during estrus cycle was studied by immunohistochemical SP method.【Result】 In endometrium layer,most NPY immunoreactive products are mainly located in cells,small parts mainly in punctuate and filamentous NPY immunoreactive fibers.In myometrium layer,The NPY immunoreactive products are mainly located in muscle fibers.The relative expression of immunoreactive product NPY of t test showed that,compared with the the proestrus,expression of NPY in estrus and diestrus of endometrium was significantly decreased（P＜0.05);Contrast with the the metestrus,estrus and diestrus were extremely significantly decreased（P＜0.01）;compared with the the estrus,expression of NPY in metestrus of myometrium was significantly increased（P＜0.05）;compared with the the metestrus,expression of NPY in diestrus of myometrium was extremely significantly decreased（P＜0.01）.【Conclusion】 The results showed that the expression of NPY in the uterus in different periods was found to adapt to some certain rules and might be related to its physiological function. |
| Key words: Neuropeptide Y immunohistochemical SP method estrus cycle rat uteru |
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