| 摘要: |
| 【目的】 从自由基生物学角度探讨迷迭香叶提取物的清除自由基、抗氧化作用，为迷迭香在食品、保健和医药等领域的进一步开发利用提供依据。【方法】 分别以水、乙醇和乙酸乙酯为溶剂，提取沪产迷迭香叶的非精油组分，然后用超氧阴离子自由基（O-·2）、羟自由基（·OH）、过氧亚硝基阴离子自由基（ONOO-）和·OH氧化损伤DNA的化学发光体系，及脂质过氧化和二苯代苦味肼基自由基(DPPH·)比色体系，检测质量浓度不同的3种提取物对自由基的清除作用和抗氧化作用，并通过计算IC50比较三者的作用效果。【结果】 迷迭香叶非精油组分3种提取物均能有效清除O-·2、·OH、ONOO-和DPPH·，减轻自由基对DNA的氧化损伤，抑制脂质过氧化，且提取物质量浓度越大，清除自由基和抗氧化的效果越好。其中水提取物抑制自由基对DNA的氧化损伤和脂质过氧化能力较强，其抑制化学发光50%时的样品质量浓度（IC50）分别为32.3和36.11 μg/mL；乙醇提取物清除O-·2和ONOO-的效果较佳，IC50分别为189.19和1.67 μg/mL；乙酸乙酯提取物清除·OH和DPPH·的效果较佳，IC50分别为4.12和49.55 μg/mL。【结论】 迷迭香叶非精油组分可有效清除自由基和抗氧化，是一种多功能自由基清除剂和天然抗氧化剂。 |
| 关键词: 迷迭香 非精油组分 自由基 抗氧化剂 |
| DOI： |
| 分类号: |
| 基金项目:上海市科学技术委员会项目（06DZ22110） |
|
| Study on scavenging free radical and antioxidant effects of nonessential oil in Rosmarinus officinalis L.leaves |
|
XIAO Lu-ping,CHEN Ji-wu,KONG Jing-si,FANG Xian-ping,GAO Qiu-yue,LI Hai-yan and YAO Lei,XIAO Lu-ping,CHEN Ji-wu,KONG Jing-si,FANG Xian-ping,GAO Qiu-yue,LI Hai-yan and YAO Lei,XIAO Lu-ping,CHEN Ji-wu,KONG Jing-si,FANG Xian-ping,GAO Qiu-yue,LI Hai-yan and YAO Lei,XIAO Lu-ping,CHEN Ji-wu,KONG Jing-si,FANG Xian-ping,GAO Qiu-yue,LI Hai-yan and YAO Lei,XIAO Lu-ping,CHEN Ji-wu,KONG Jing-si,FANG Xian-ping,GAO Qiu-yue,LI Hai-yan and YAO Lei,XIAO Lu-ping,CHEN Ji-wu,KONG Jing-si,FANG Xian-ping,GAO Qiu-yue,LI Hai-yan and YAO Lei,XIAO Lu-ping,CHEN Ji-wu,KONG Jing-si,FANG Xian-ping,GAO Qiu-yue,LI Hai-yan and YAO Lei
|
| Abstract: |
| 【Objective】 From free-radical biology angle，the effects of nonessential oil extracts of Rosmarinus officinalis L.leaves on scavenging free radical and antioxidant effects were studied to provide a basis for its development in food,healthcare and medical etc.【Method】 We used water,grain alcohol and ethyl acetate as solvent to extract nonessential oil components from R.officinalis L.leaves in Shanghai,then detected scavenging free radical and antioxidant effects of three different mass concentration extracts using chemiluminescence systems of superoxide anion free radical,hydroxyl radical,peroxynitrite and DNA oxidative damage caused by hydroxyl radical and colorimetric systems of lipid pexoxidation and DPPH free radical.The effects of three extracts were compared by calculating the IC50.【Result】 Three extracts of R.officinalis L.can effectively scavenge O-·2,·OH,ONOO- and DPPH·,reduce DNA oxidative damage caused by free radical and inhibit lipid pexoxidation.And it showed that the extract concentration was higher and the effect better.Deionized water extraction had better effect on reducing DNA oxidative damage caused by free radical and inhibitting lipid pexoxidation and the IC50 was 32.3 μg/mL，36.11 μg/mL respectively;grain alcohol extraction had better effect on scavenging O-·2 and ONOO-,and the IC50 was 189.19 μg/mL，1.67 μg/mL respectively;ethyl acetate extraction had better effect on scavenging ·OH and DPPH·,and the IC50 was 4.12 μg/mL，49.55 μg/mL respectively.【Conclusion】 Extracts of R.officinalis L.that can effectively scavenge free radical and resist oxidation are multifunctional free radical scavenger and natural antioxidants. |
| Key words: Rosmarinus officinalis L. nonessential oil free radical antioxidant |
