| 摘要: |
| 【目的】 应用白腐真菌处理造纸黑液，探讨白腐真菌对造纸黑液中木质素的降解效果及其影响因子，确定最佳降解条件。【方法】 从自然界腐朽木材上分离培养白腐真菌，利用专用培养基对其进行分离和纯化，分析木质素溶液质量浓度（污染负荷）、pH值、搅拌速度、碳源和氮源质量浓度、［Fe2+］质量浓度对白腐真菌降解木质素效果的影响。【结果】 确定白腐真菌降解造纸黑液中木质素的最佳条件为：木质素质量浓度为153.0 mg/L，pH值为5，搅拌速度为250 r/min，葡萄糖质量浓度为1 g/L，酒石酸铵质量浓度为0.20 g/L，［Fe2+］质量浓度为0.08 mg/L。【结论】 得到了白腐真菌降解造纸黑液中木质素的最佳条件，在此条件下白腐真菌对木质素的降解率最高可达64.38%。 |
| 关键词: 白腐真菌，木质素，生物降解，造纸黑液 |
| DOI： |
| 分类号: |
| 基金项目:陕西省教育厅重大产业化项目(05JC15) |
|
| Study on factors influencing the degradation of lignin in black liquor by white-rot fungus |
|
WANG Ya hong,WANG Zheng zhong,LI Xiao hui，YAO Ru fang,WANG Ya hong,WANG Zheng zhong,LI Xiao hui，YAO Ru fang,WANG Ya hong,WANG Zheng zhong,LI Xiao hui，YAO Ru fang
|
| Abstract: |
| 【Objective】 The study chose the white-rot fungus to process black liquor to explore the degradability and the influence factors of white-rot fungus on lignin of black liquor.【Method】 Special culture medium was used to separate and purify white-rot fungus which had been separated from the rotten wood and cultured.The influence of degradation was analyzed by the following ingredients:lignin consistency,pH value,stirring speed,consistency of carbon and nitrogen source,and Fe2+ consistency,so as to choose the best conditions of white-rot fungi for degradation.【Result】 The best condition of degrading black liquor is determined:lignin=153 mg/L,pH=5,the rotating speed is 250 r/min,glucose=1 g/L,ammonium tartrate=0.20g/L,Fe2+=0.08mg/L.【Conclusion】 The best degradation conditions in which white-rot fungus degrade black liquor are obtained.Under these conditions,the degradation rate of white-rot fungus is up to 64.38%. |
| Key words: white-rot fungus lignin biodegradable degradation of black liquor |
