| 摘要: |
| 【目的】 确定ABTS·+法测定葡萄酒抗氧化活性的最佳反应时间和葡萄酒的最佳稀释倍数。【方法】 应用福林肖卡比色法（FC）测定36种葡萄酒样品的总酚含量(Total phenol index，TPI),从中选出9种总酚含量具代表性的葡萄酒样品，并采用ABTS·+法测定反应不同时间和稀释不同倍数葡萄酒样品的抗氧化活性。【结果】ABTS·+法测定葡萄酒抗氧化活性的最佳反应时间为2～5 min；红葡萄酒的最佳稀释倍数为0.2∶10～0.4∶10，桃红葡萄酒的最佳稀释倍数为1∶10～4∶10，白葡萄酒的最佳稀释倍数为3∶10～7∶10。【结论】 得到了ABTS·+法测定葡萄酒抗氧化活性的最佳反应时间和葡萄酒的最佳稀释倍数，且无需事先测定总酚含量，简化了试验步骤。 |
| 关键词: 葡萄酒 抗氧化活性 ABTS·+法 反应时间 稀释倍数 |
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| 基金项目:陕西省“13115”科技创新工程重大科技专项“陕西省葡萄与葡萄酒产业关键研究” （2007ZDKG-09） |
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| Research of antioxidant activity of wines determined by the ABTS·+ method |
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LIU Hui,ZHANG En hui，XU Zhong min，CHENG Yong an，LI Hong wei,LIU Hui,ZHANG En hui，XU Zhong min，CHENG Yong an，LI Hong wei,LIU Hui,ZHANG En hui，XU Zhong min，CHENG Yong an，LI Hong wei
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| Abstract: |
| 【Objective】 The present study aimed to confirm the best reaction time and wine dilution of the ABTS·+ method.【Method】 The Folin Ciocalteu colorimetry was used to measure the total phenol index of 36 kinds of wines and then 9 kinds of representative samples were selected from them, ABTS·+ method was used to measure the antioxidant activities of wines with different reaction time and different dilutions,and was analyzed the experiment result.【Result】 The results showed that the best reaction time of the ABTS·+ method is 2 to 5 minutes,the best dilution range,0.2∶10 to 0.4∶10 for red wines,1∶10 to 4∶10 for rose wines,and 3∶10 to 7∶10 for white wines.【Conclusion】 The study has obtained the best reaction time and wine dilution to measure antioxident activity of wines by ABTS·+ method,and there is no need to measure total phenol content beforehand,so the test procedure is simplified. |
| Key words: wine antioxidant activity ABTS·+ method reaction time dilution |
