| 摘要: |
| 【目的】 分析获能与冻融猪精子细胞肌动蛋白表达的差异。【方法】 提取获能和冻融的猪精子蛋白，经过SDS-PAGE分离后，进行Western-blot免疫印迹分析，检测获能与冻融猪精子间细胞肌动蛋白的区别。【结果】 新鲜和冻融猪精子细胞肌动蛋白单体的分子质量均约为43 ku，但获能猪精子细胞肌动蛋白单体的分子质量约为43和86 ku。【结论】 冻融的猪精子细胞肌动蛋白单体分子质量约为43 ku，但数量有所下降；获能后的猪精子出现了一种新型肌动蛋白亚单位，其分子质量约为86 ku。 |
| 关键词: 猪精子 获能 冻融 肌动蛋白 |
| DOI： |
| 分类号: |
| 基金项目:“211”工程重点学科项目；延边大学研究生科研项目 |
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| Difference between capacitation and frozen-thawed cell actin expression in boar sperm |
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SONG Zhao peng1,2,JI Xin wei3,WANG Sheng lei4,
ZHANG Yong gang1,GONG Chang rong1,SONG Zhao peng1,2,JI Xin wei3,WANG Sheng lei4,
ZHANG Yong gang1,GONG Chang rong1,SONG Zhao peng1,2,JI Xin wei3,WANG Sheng lei4,
ZHANG Yong gang1,GONG Chang rong1
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| Abstract: |
| 【Objective】 The study was done in order to analyze the difference between capacitation and frozen-thawed boar sperm cells actin expression.【Method】 After SDS-PAGE separation of the capacitation and frozen-thawed boar sperm,western blotting analysis was carried out to detect the distinction between capacitation and frozen-thawed boar sperm cell actin.【Result】 Monomer molecular weight of fresh and frozen-thawed boar sperm cells actin was about 43 ku,but capacitation boar sperm cells actin was about 43 ku and 86 ku.【Conclusion】 Monomer molecular weight of frozen-thawed boar sperm cells actin was still 43 ku,but there was a decrease in number;a new actin subunit appeared in capacitation boar sperm,and the molecular weight was 86 ku . |
| Key words: boar sperm capacitation frozen-thaw actin |
