| 摘要: |
| 【目的】 定量实时检测奶牛牛乳中的乳酶活性，通过分析其变化规律为奶牛隐性乳房炎的诊断提供理论依据。【方法】 随机选择220头荷斯坦泌乳奶牛，采集乳汁测定体细胞数(Somatic cell counts，SCC)和5种乳酶活性，研究乳酶活性在健康和患隐性乳房炎奶牛乳汁中的变化规律及与SCC的相关性。【结果】 以乳汁体细胞数50×104 mL-1作为判定乳房炎的标准，患隐性乳房炎奶牛乳汁中N-乙酰基-1-β-D-氨基葡萄糖苷酶(NAG)、乳酸脱氢酶(LDH)、乳过氧化物酶(LP)、碱性磷酸酶(ALP)和髓过氧化物酶(MPO)活性均显著高于健康奶牛(P<0.05)，乳酶活性与SCC(lg SCC)呈正相关，其中NAG活性与SCC(lg SCC)的相关性系数R2=0.98，NAG与LDH活性之间的相关性系数R2=0.95。【结论】 奶牛隐性乳房炎对LDH、NAG、LP、ALP和MPO活性有显著影响(P<0.05), 乳酶NAG和LDH可联合应用作为早期奶牛隐性乳房炎的诊断指标。 |
| 关键词: 奶牛 隐性乳房炎 乳酶活性 体细胞计数 |
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| 基金项目:国家自然科学基金项目(30571329)；教育部长江学者和创新团队发展计划创新团队项目（IRT0848）；四川农业大学青年科技创新基金项目（00231800） |
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| Enzymes in cow milk as diagnostic indicator of subclinical mastitis |
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| Abstract: |
| 【Objective】 To provide theoretical basis of diagnostic subclinical bovine mastitis,activity of enzyme was detected in quantization and real time.【Method】 To study the variations of N-acetyl-β-D-glucosaminase(NAG),lactate dehydrogenise(LDH),myeloperoxidase(MPO),alkaline phosphatase(ALP) and lactoperoxidase(LP) between health and subclinical mastitis cows,the milk samples were collected from 220 lactating Holstein cows in one dairy farm randomly.Somatic cells were counted (SCC) and activities of five enzymes were measured in milk samples.【Result】 A SCC threshold of 50×104 mL-1 in milk was used as the classification standard of subclinical mastitis.Activities of five milk enzymes in cows with subclinical mastitis were higher than the healthy significantly (P<0.05) and were positively correlated with SCC (lg SCC ).Correlation coefficient was 0.98 between lg SCC and activity of NAG,and 0.95 between activity of NAG and LDH.It was concluded that subclinical mastitis influenced the activities of five milk enzymes significantly (P<0.05).【Conclusion】 NAG and LDH could unite as the indicator in the earlier diagnosis of bovine subclinical mastitis. |
| Key words: cow subclinical mastitis activity of milk enzyme somatic cell count (SCC) |
