| 摘要: |
| 【目的】 检测78株油樟内生真菌（其中根内生真菌43株，茎内生真菌11株，叶内生真菌24株）代谢产物的组分。【方法】 78株油樟内生真菌经PDA液体培养基摇床培养8 d后，取液体发酵产物，采用GC MS法检测其乙醚提取液中的挥发性物质。【结果】 多数油樟内生真菌均可产C10~C28之间的烷烃（二十烷最常见）和C16~C19脂肪酸及其对应的甲酯；55株内生真菌产烷基苯；35株内生真菌可产叔丁基苯酚的多种同分异构体；70株内生真菌发酵液中都检测到α-萜品醇，其中2株相对含量较高；5株内生真菌发酵产物中可以检测到黄樟油素；4株内生真菌发酵产物中可以同时检测到黄樟油素及桉叶油素。另有2株内生真菌的主要产物分别是蘑菇醇及苯乙酸。【结论】 油樟内生真菌与油樟之间，内生真菌相互之间存在广泛的醇类物质共代谢；油樟内生真菌代谢产物对油樟植株具有促生、抗氧化、协助合成活性物质等作用；某些油樟内生真菌能够合成一些特殊产物，具有一定的开发利用价值。 |
| 关键词: 油樟 内生真菌 GC-MS |
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| 基金项目:四川省科技厅应用基础研究项目（07JY029-001）；四川省教育厅青年基金项目（2006B079）；国家自然科学基金项目（30660004，30460004，30260004）；宜宾学院青年基金项目（2007Q19） |
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| Analyses on volatile organic compound of 78 endophytic fungi isolated from Cinnamomum longepaniculatum N.Chao ex H.W.Li |
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| Abstract: |
| 【Objective】 This study explored the metabolic constitution to of 78 endophytic fungi(43 were from roots,11 from stems,and 24 from leaves)isolated from Cinnamomum longepaniculatum N.Chao ex H.W.Li.【Method】 78 endophytic fungi were cultured for 8 d in PDA liquid medium.The productions were extracted by absolute ethyl ether, and then gas chromatography/mass spectrometry (GC-MS) method was used to detect their volatile organic compounds.【Result】 Most strains can produce C18H38N2O, alkanes of C10-C28 (C20 generally) and a lot of fatty acids or fatty acid methyl esters of C16-C19;55 strains produced alkyl benzene,and 35 strains 2-butyl phenol;70 strains produced terpineol,of which two strains produced in large quantities;5 strains produced safrole;4 strains produced cineole and safrole simultaneously;2 strains produced morillol and phenylacetic acid respectively.【Conclusion】 Comprehensive co-metabolism of alcohol exists among endophytic fungi and Cinnamomum longepaniculatum N.Chao ex H.W.Li;Endophytic fungi can promote the growth of Cinnamomum longepaniculatum N. Chao ex H.W.Li,and help the host in antioxidation and synthesis of bioactive metabolites;Some microbes with potential value for application have been obtained in the study. |
| Key words: Cinnamomum longepaniculatum N.Chao ex H.W.Li endophytic fungi GC-MS |
