| 摘要: |
| 【目的】 研究AG490阻断肥胖模型小鼠JAK2/STAT3信号通路对脂代谢及相关基因表达的影响。【方法】 以雄性昆明小鼠为供试动物，构建肥胖模型。将肥胖模型小鼠分为2组，处理组用JAK2特异性抑制剂AG490(1 mg/(kg·d))腹腔连续注射2周，对照组腹腔连续注射相同剂量的生理盐水。2周后处死小鼠，检测各组小鼠血清中总胆固醇（TC）、总甘油三酯（TG）、高密度脂蛋白固醇（HDL-C）的含量；取脂肪组织提取总RNA，RT-PCR方法检测JAK2/STAT3信号通路基因（JAK2,STAT3）及脂代谢关键基因（FAS,PPARγ,HSL）的表达量。【结果】 与对照组相比，AG490可显著降低小鼠血清中的HDL-C水平；与对照组相比，处理组脂肪组织中JAK2 mRNA表达量差异不显著（P＞0.05），STAT3 mRNA表达量显著降低（P＜0.05），脂代谢相关基因FAS mRNA表达量极显著降低（P＜0.01），PPARγ mRNA表达量显著降低（P＜0.05），HSL mRNA表达量差异不显著（P＞0.05）。【结论】 JAK2/STAT3通路可能通过影响脂肪组织中生脂基因的表达而调节体脂沉积。 |
| 关键词: JAK2 STAT3 AG490 肥胖模型 脂代谢 |
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| 基金项目:国家自然科学基金项目（30871785）；国家“十一五”支撑计划项目（2006BAD04A11）；教育部新世纪优秀人才计划项目 （NCET-06-0865） |
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| Effects of AG490 on lipid metabolism of the obese mouse and expression of related genes |
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| Abstract: |
| 【Objective】 It aimed to study the effects of AG 490 on lipid deposition and expression of relevant genes of the obese mouse by JAK2/STAT3 signal transduction pathway occlusion. 【Method】 The obese model which was constructed by the male Kunming mouse were divided into 2 groups.The treatment group was treated with intraperitoneal injection by AG490 (1 mg/(kg·d)) and the control group was treated by the same method with NS (1 mg/(kg·d)) for two weeks,then executed and the adipose was reserved.To estimate the content of TC,TG and HCL-C in blood,Total RNA was extracted. RT-PCR was used to analyze the expression of JAK2/STAT3 signal transduction pathway and adipogenesis genes (FAS, PPARγ and HSL).【Result】 Compared with the control group, AG490 was able to cut down the level of HDL-C significantly in blood with the treated mice;Compared with the control group,the mRNA expression of JAK2 had not significant difference (P＞0.05) and the mRNA expression of STAT3 was significantly lowered (P＜0.05) in the treatment group.The mRNA expression of FAS was significantly lower (P＜0.01),the mRNA expression of PPARγ had a significant reduction (P＜0.05) and the mRNA expression of HSL had no significant difference (P＞0.05).【Conclusion】 We conjectured that JAK2/STAT3 signal transduction pathway regulated lipid deposition by affecting the expression of adipogenesis genes in adipose tissue. |
| Key words: JAK2 STAT3 AG490 obesity model lipid metabolism |
