| 摘要: |
| [目的]探明宁夏地区葡萄自然发酵过程中酵母菌的多样性,为酵母菌资源的开发利用提供依据.[方法]应用26S rDNA D1＼D2区序列,分析鉴定了分离自宁夏广夏三基地葡萄园的21株野生葡萄酒酵母菌,在此基础上又构建了21株被测菌株与相关菌种模式株的系统发育树,分析了供试菌株与已知酵母菌的亲缘关系及其分类地位.[结果]序列分析和系统发育树分析表明,21株被测菌株鉴定为6属7种,分别为拜耳接合酵母(Zygosaccharomyces bailii)、酿酒酵母(Saccharomyces cerevisiae)、罕见有孢汉生酵母(Hanseniaspora occidentalis)、葡萄汁有孢汉生酵母(Hanseniaspera uvarum)、美极梅齐酵母(Metschnikowia pulcherrima)、东方伊萨酵母(Issatchenkia orientali)和克鲁维毕赤酵母(Pichia kluyveri).[结论]宁夏葡萄酒产区的相关酵母具有多样性. |
| 关键词: 野生葡萄 酿酒酵母 自然发酵 生物学鉴定 |
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| 基金项目:西北农林科技大学青年骨干支持计划项目(01140301) |
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| Molecular identification of wild wine-related yeasts isolated from spontaneous wine fermentation in Ningxia district |
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| Abstract: |
| 【Objective】 The study was done to reveal the diversity and the composition of the yeast so as to provide supports for development and utilization of yeast. 【Method】 21 wild yeasts isolated from Guangxia Third vinayard of Ningxia were identified by using 26S rDNA D1/D2 domain sequences.Phylogentic tree was constructed to study the relationship and confirm the classification status of the yeast strains. 【Result】 As a result,7 species belonging to 6 genera were recognized.They were Zygosaccharomyces bailii,Saccharomyces cerevisiae,Metschnikowia pulcherrima,Pichia kluyveri,Hanseniaspera uvarum,Hanseniaspora occidentalis,Issatchenkia orientali. 【Conclusion】 The result reveals the diversity of wine related wild yeasts in wine district of Ningxia. |
| Key words: wild grape wine-related yeast spontaneous fermentation molecular identification |
