| 摘要: |
| [目的]为重金属污染区的生物修复提供科学依据.[方法]采用含不同浓度K2CrO4(0(对照),0.4,0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗,测定Cr在黑麦体内的亚细胞分布、Cr的化学形态及其不同部位的积累.[结果]黑麦幼苗地上部和根部Cr含量随Cr处理浓度的增加而显著增大,并且根部的Cr含量均明显大于地上部.0.4,0.8和1.2 mmol/L Cr处理黑麦幼苗地上部Cr含量分别是对照的3.3,27.8和29.5倍,其根部Cr含量分别是对照的8.8,18.7和19.6倍.Cr在亚细胞中的分布表现为F1(细胞壁及残渣)>F3(可溶性部分)> F2(细胞器及膜部分).不同处理黑麦地上部和根部F1(细胞壁及残渣)中的Cr含量所占比率均在50%以上.1 mol/L NaCl、体积分数2%醋酸和0.6 mol/L盐酸3种提取态Cr的比例较高.[结论]黑麦对Cr的吸收主要积累在根部和细胞壁中,Cr在黑麦体内以多种形态存在,其中在盐酸提取态的草酸盐类Cr含量相对较高. |
| 关键词: 黑麦 铬 亚细胞分布 铬化学形态 |
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| 基金项目:国家自然科学基金项目（30471184) |
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| Absorption and distribution of chromium in rye seedling |
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| Abstract: |
| 【Objective】 The research about the absorption, distribution and accumulation of Cr in rye seedlings provides the theoretical basis for the phytoremediation of heavy metals in contaminated areas.【Method】 The different concentrations of K2CrO4 in Hoagland solution were adopted to study the accumulation,subcellular distribution and chemical forms of chromium in rye seddlings. 【Result】 The results obtained show that the contents of chromium in aerial parts and roots increased,accompanying with the increment of concentration of K2CrO4,and more in roots than aerial parts.With the different treatments of 0.4,0.8 and 1.2 mmol/L Cr,the contents of chromium in aerial parts were 3.3,27.8,and 29.5 times respectively as much as in the control,and 8.8,18.7,and 19.6 times in roots.Chromium was mainly distributed in F1(cell wall and residue) and F3(soluble fraction),the distribution ratio of chromium in F2(cell organs and membrane) was the last.There were different distribution ratios of chromium in different extraction media,NaCl extractable-Cr,Acetic acid extractable-Cr and HCl extractable-Cr were dominant in roots and aerial parts in rye.【Conclusion】 Cr accumulates mainly in the cell wall and the roots in rye,and there are varieties of forms in the plant,the content of HCl extractable-Cr is higher than that of others. |
| Key words: Secale cereale Cr subcellular distribution chemical form of Cr |
