| 摘要: |
| [目的]筛选体外培养昆明小鼠原始生殖细胞(PGCs)的最佳无血清培养基,为昆明小鼠胚胎生殖细胞(EGCs)建系奠定基础.[方法]以妊娠8.5～12.5 d昆明小鼠PGCs为材料,采用无血清培养基,在培养基中添加不同组分,组成A、B、C、D、E、F、G 7种培养基,对昆明小鼠PGCs进行体外培养,分离培养胚胎原始生殖细胞,以筛选出其最佳培养基.[结果]D、E、F组均能得到较多的小鼠PGCs阳性集落.MEF-CM和GR-CM组的效果与细胞因子组相比差异不显著,但降低了培养基的成本.[结论]在无血清培养基中添加0.1 mmol/L RA或使用条件培养基MEF-CM和GR-CM,均有利于小鼠PGCs增殖.经过EGCs鉴定,本试验得到的细胞是PGCs,并且在各组培养基中均能够形成EGCs集落. |
| 关键词: 原始生殖细胞 胚胎生殖细胞 无血清培养基 昆明小鼠 |
| DOI： |
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| 基金项目:国家自然科学基金项目（30671067）；陕西省重大项目（2006K205-G1） |
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| Selection of serum-free medium for culturing primordial germ cells of Kunming mice |
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| Abstract: |
| 【Objective】 The study was to choose the best serum-free medium for culturing Kunming mice PGCs in vitro.【Method】 Groups of mediums,A，B，C，D，E，F and G with different components were used to separate and culture PGCs from 8.5-12.5 days'fetuses.【Result】 D，E and F groups could culture more positive PGCs colonies.Comparison between groups of MEF-CM and GR-CM with group of cell factors didn't show dignificant differences,but they decreased the cost of mediums.【Conclusion】 The mediums with 0.1 mmol/L RA or MEF-CM and GR-CM were helpful for proliferation of mouse PGCs.After EGCs were characterized,the cells were proved to be PGCs,and EGCs colonies were obtained in each group of medium. |
| Key words: primordial germ cell embryonic germ cell serum-free medium Kunming mouse |
