| 摘要: |
| [目的]了解健康朱鹃消化道正常菌群,为进一步研制适用于朱鹦的微生态制剂奠定基础性.[方法]无菌采集健康朱鹃的新鲜粪便,分离、培养、鉴定其细菌.以小白鼠和小鸡为试验动物,对分离、鉴定出的细菌进行毒性试验,确定分离的细菌对动物是否有致病性.[结果]从朱鹃粪便中分离得到22株细菌,分别为蜡状芽孢杆菌、环状芽孢杆菌、凝结芽孢杆菌、泛酸芽孢杆菌、两歧双歧杆菌、最小双歧杆菌、口乳杆菌、短乳杆菌、面包乳杆菌、马里乳杆菌、瑞士乳杆菌、嗜酸乳杆菌、丙酸丙酸杆菌、粪肠球菌、类肠膜明串球菌、少酸链球菌、肠膜链球菌、乳链球菌、克雷伯菌属、柠檬酸菌属,变形菌属和粪拟杆菌.其中,环状芽孢杆菌和泛酸芽孢杆菌可致小白鼠和小鸡死亡;短乳杆菌可致小白鼠死亡,但对小鸡无致病性;其他菌株对小白鼠和小鸡无致病性.[结论]分离鉴定出的朱鹮消化道22株细菌,其中适合制作微生态制剂的细菌有两歧双歧杆菌、最小双歧杆菌、口乳杆菌、面包乳杆菌、马里乳杆菌和嗜酸乳杆菌. |
| 关键词: 朱鹮 消化道 正常菌群 分离鉴定 |
| DOI： |
| 分类号: |
| 基金项目:陕西省2003年林业科学研究课题 |
|
| Isolation and Identification of normal flora in healthy Nipponia Nippon enteron |
|
|
| Abstract: |
| 【Objective】 The research was to know normal flora in healthy Nipponia Nippon enteron in order to provide basis for ibis’microecologic preparation.【Method】 Bacteria of fresh feces of Nipponia Nippon were cultured.According to different colonies from the solid culture mediums,they were isolated,purified and confirmed through microscopic observation and then identified tests were carried out.After that challenged tests were taken to detect germs’pathogenicity in animals.【Result】 30 strains of bacteria firstly were isolated.Finally,22 strains of bacteria were identified by biochemistry tests.They were B.cereus，B.circulans，B.coagulans，B.pantothenticus，B.bifidum，B.mininmum，L.oris，L.brevis，L.panis，L.mali，L.helvericus，L.acidophicus，P.acidpropionici，E.faecalis，L.paramesenteroides，S.acidominimus，S.intestinalis，lactococcus，klebsiella，citrobater，proteus and B.caccae.The results of challeged test to all isolated strains showed that B.circulans and B.pantothenticus were pathogentic to animals apparently;other isolated germs had no evident pathogentic effect on animals.【Conclusion】 22 strains of bacteria of Nipponia Nippon enteron were isolated and identified,of which the bacteria used to make microecologic preparion are B.bifidum，B.mininmum，L.oris，L.panis，L.mali，L.acidophicus and et al.And these provide very valueable materials for more research on microecologic preparion. |
| Key words: Nipponia Nippon enteron normal flora isolation and identification |
