| 摘要: |
| [目的]探讨牛Dmrt7基因的生物学功能,为进一步研究该基因对牛精液品质的影响奠定基础.[方法]以牛睾丸组织为材料,根据GenBank中发表的小鼠Dmrt7基因序列,结合生物信息学技术,设计并合成2对引物,采用RT-PCR方法克隆牛Dmrt7 cDNA,并运用DNAMAN软件及在线工具对所得到的序列进行了生物信息学分析.采集牛肾脏、肝脏、睾丸、肺、脾脏、瘤胃、子宫、小肠、心脏、卵巢和肌肉等11个组织,以牛β-actin基因为内参基因,对牛Dmrt7基因的组织表达谱进行分析.[结果]牛Dmrt7基因cDNA长度为1 378 bp(Genbank登录号为EF534775),包含由1113个碱基组成的开放读码框(ORF),该ORF包含1个TAG终止密码子,编码370个氨基酸;在进化关系上,牛首先与狗聚为一类,亲缘关系最近,再与小鼠和大鼠聚为一类,最后与人和短尾猿聚为一类.Dmrt7蛋白含有一个DM结构域.牛Dmrt7基因在睾丸组织中高度表达,在其他组织中不表达.[结论]获得了牛Dmrt7基因的cDNA序列(Genband登录号为EF534775);牛Dmrt7蛋白可能对精液品质有影响. |
| 关键词: 牛；Dmrt7基因； cDNA 克隆；生物信息 |
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| 基金项目:国家“十一五”科技支撑计划重大项目“农林动植物育种工程”（2006BAD01A10）；国家“十一五”高技术研究发展计划项目(2006AA10Z197) |
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| The cDNA cloning and biological information analysis of cattle Dmrt7 |
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| Abstract: |
| 【Objective】 This paper will discuss biological function of Dmrt7 gene,which is a basis to study bovine sperm quality.Bovine kidney,liver,testicle,lung,spleen,rumen,uterus,small intestine,heart,ovary and muscle tissues were used as experimental materials. 【Method】 Two pairs of primers were designed according to the sequence of the mouse Dmrt7 gene sequence published in GenBank and combining bioinformatics technology to amplify the Dmrt7 gene.RT-PCR technique was used to amplify the cDNA sequence of Dmrt7 gene.The cDNA sequence of Dmrt7 gene was analyzed by using the DNAMAN software and online tool.At the same time,one pair of primer was designed;the control primer was designed according to the bovine β-actin gene.These two pairs of primers were to amplify the Dmrt7 gene in kidney,liver,testicle,lung,spleen,rumen,uterus,small intestine,heart,and ovary and muscle tissues.【Result】 The result showed that the sequence of cDNA was 1 378 bp (GenBank Accession No.EF534775) containing an open reading frame (ORF) of 1 113 bases which encoded a 370 amino acid and 1 TAG termination codon.Phylogenetic tree of amino acid analysis indicated that cattle and dog gathered in one sort,the evolvement relation was close.Cattle,mouse and rat gathered again in one sort.Finally,cattle,human and macaque gathered in one sort.One DM domain was contained in Dmrt7 protein through the bioinformatics analysis.The result showed that Dmrt7 was found in testicle and none in other tissues.【Conclusion】 The cDNA of Dmrt7 gene was obtained;Dmrt7 gene probably affects sperm quality. |
| Key words: cattle Dmrt7 gene cDNA cloning biological imformation |
