| 摘要: |
| [目的]从酶学角度揭示好氧发酵的生物机理.[方法]选择在目前我国数量多、分布广的有机固体废弃物鸡粪和小麦秸秆及花椒籽粕为主要材料,采用堆腐装置,以未添加复合微生物菌剂作为对照(CK),研究复合微生物菌剂(加菌剂处理)对农业废弃物静态高温堆腐过程中氧化还原酶(过氧化氢酶、脱氢酶和多酚氧化酶)活性的影响.[结果]加菌剂处理和CK的过氧化氢酶活性分别在堆腐3 和4 d达到峰值(1 705和1 697 mL/g );加菌剂处理和CK的脱氢酶活性分别在堆腐10和12 d达最大值(8.39和6.57 μL/g);加菌剂处理和CK的多酚氧化酶活性分别在堆腐18和32 d达峰值(7.33和6.65 mg/g).从堆腐的整个过程看,随着堆腐时间的延长,加菌剂处理的过氧化氢酶、脱氢酶和多酚氧化酶活性上升较快,而且这3种酶活性的最大值均比CK高.[结论]添加复合微生物菌剂可以提高堆腐过程中氧化还原酶活性,使酶活性峰值出现的时间提前,对堆腐过程中的物质分解具有促进作用. |
| 关键词: 复合微生物菌剂 农业废弃物 堆腐 氧化还原酶活性 |
| DOI： |
| 分类号:F270.5 |
| 基金项目:国家自然科学基金项目(40771109);陕西省农业科技攻关项目(2004K012G15202,2005K042G11);科技部农业科技成果转化项目(05EFN 21601195) |
|
| Activity changes of oxidoreductase during composting of agricultural waste materials |
|
CHEN Bi-an,CHEN Bi-an,CHEN Bi-an
|
| Abstract: |
| 【Objective】 An experiment was conducted with enzymology theory and analysis technique to study dynamic change of enzyme activity in the static temperature composting process to reveal further biological mechanism during the aerobic fermentation with oxygen.【Method】 During the aerobic composting process of the agricultural waste material,microorganism plays an essential role.This experiment was conducted with a large amount of materials from chicken excrement and wheat straw in a wide range in China to study the activity changes of oxidoreductase during composting at high temperature and in a static state．【Result】 The result showed temperatures of different positions of the treatments adding microorganism agent were higher than those of CK，and the temperatures rose more quickly than those of CK.The activity of enzyme of H2O2 in the materials adding microorganism agent reached the peak value by the third day 1 705 mL/g,while that of CK reached the peak value by the fourth day 1 697 mL/g;dehydrogenase activity,reached the peak value by the tenth day 8.39 μL/g under the condition of adding microorganism agent, and the value of that of CK was by twelfth day 6.57 μL/g;the peak value of polyphenol oxidized activity was up to 7.33 mg/g by the eighteenth day,while that in CK was 6.65 mg/g by the 32 days.Through the study we could know enzyme under the condition of adding microorganism agent rose quickly and moreover the maximum of enzyme was also higher than the value of that in CK.The activity of the enzyme of H2O2 was higher than CK in a basic level during the whole composting process.The dehydrogenase activity with microorganism agent in the middle period of composting was higher than CK.The polyphenol oxidized activity,26 days before composting was higher than those in CK.【Conclusion】 It is found that adding microorganism agent could improve the activity of oxidoreductase,promote the decomposition of composting,and promote degradation of lignin and its transformation. |
| Key words: microorganisms agent agricultural waste materials compost activity of oxidoreductase |
