| 摘要: |
| 克隆猕猴桃果实L-半乳糖内酯脱氢酶（GalLDH）和脱氢抗坏血酸还原酶(DHAR)基因,可为揭示猕猴桃高抗坏血酸（AsA）含量的分子机制奠定基础。研究以猕猴桃果实为材料提取总RNA，采用 RT-PCR 法，克隆出L-GalLDH基因的cDNA片段 857 bp和DHAR基因的cDNA片段594 bp并测序，根据测序结果推测其氨基酸序列，并与刺梨、拟南芥、花椰菜、甘薯和草莓等植物的GalLDH基因和DHAR基因氨基酸序列进行亲缘分析。结果表明，猕猴桃GalLDH基因氨基酸序列和核苷酸序列与刺梨的相似性高达88％,其在同源基因进化关系树中单独聚为一类；DHAR基因氨基酸序列和核苷酸序列与苹果的相似性高达98％和99％，两者在进化关系上聚为一类。可知克隆到的2个核苷酸片段确为猕猴桃L-GalLDH和DHAR基因cDNA 片段,同源基因植物亲缘关系聚类与传统的形态分类有一定的差异。 |
| 关键词: 猕猴桃 L-半乳糖内酯脱氢酶(GalLDH) 脱氢抗坏血酸还原酶（DHAR） 维生素C 基因克隆 |
| DOI： |
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| 基金项目:陕西省重大科技专项计划项目（2006kz05-G4）；西北农林科技大学“拔尖人才支持计划”项目 |
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| Cloning and sequence analysis of cDNA fragments of L-galactono 1,4-lactone dehydrogenase and dehydroascorbate reductase from fruit of Actinidia deliciosa |
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| Abstract: |
| In order to research the molecular mechanism of Vc accumulation,857 bp and 594 bp cDNA encoding L galactono-1,4-lactone dehydrogenase（GalLDH）and dehydroascorbate reductase（DHAR） fragment were cloned from fruit of Actinidia deliciosa by the method of RT-PCR,and then translated into amino acid sequences.In GenBank compared with Rosa roxburghii Trat,Arabidopsis thaliana,Ipomoea batatas.et.homologous genes plants,the nucleotide sequence and the amino acid sequence of GalLDHgene showed 88% of identity with those of Rosa roxburghiiTratt.The nucleotide sequence and the amino acid sequence of DHARgene showed 98% and 99% of identity with those apples,respectively,and they both were classified the same family.These results suggest that the both cDNA fragments are from DHAR and GalLDH gene of Actinidia deliciosa. |
| Key words: kiwifruit L-galactono-1,4-lactone dehydrogenase (GalLDH) dehydroascorbate reductase（DHAR） ascorbic acid (AsA) gene cloning |
