| 摘要: |
| 为了获得超声波提取辅酶Q10的最佳工艺条件,系统研究了利用超声波法提取时输出功率、每次辐射时间、工作总时间、水添加量等因素对细胞破碎和辅酶Q10提取效果的影响。结果表明,超声波提取辅酶Q10的最佳工艺条件为:输出功率500 W,每次辐射/间歇时间12 s/10 s,工作总时间12 min,水添加量45 mL/g;采用优化的超声波提取工艺,辅酶Q10提取量可达到1.196 mg/g。证明超声波破碎法提取辅酶Q10是可行的,与未破壁提取、研磨法及反复冻融法提取效果相比,辅酶Q10提取效果良好。 |
| 关键词: 辅酶Q10 超声波法 细胞破碎 提取工艺 |
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| 基金项目:“十一五”国家科技支撑计划项目（2006BAK02A24）;农业部科技跨越计划项目（2005-4.1）；2005年教育部新世纪优秀人才支持计划项目；陕西省科技重大专项（2006KZ09-G1） |
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| Study on the technology of the coenzyme Q10 extraction with ultrasonic cell-break method |
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| Abstract: |
| In order to obtain the optimal condition of ultrasonic cell-break method for coenzyme Q10 extraction,the effect of output power,duration of radiation each time,total working time and amount of water on cell-wall disruption and coenzyme Q10 extraction were systematically studied in this paper.The results showed that the optimal condition for coenzyme Q10 extraction was as follows output power 500 W,radiation/intermission time 12 s/10 s,total working time 12 min,and amount of water 45 mL/g;the quantity of coenzyme Q10 extracted by ultrasonic cell-break method could reach 1.196 mg/g under the optimal condition.It's proved that the method of the ultrasonic cell-break for extracting coenzyme Q10 was feasible and more effective than the method of non-broken,grinding,freezing and melting repeatedly. |
| Key words: coenzyme Q10,ultrasonic method,cell break,extraction technology |
