| 摘要: |
| 以A型流感病毒NP基因为靶标,设计并合成了3对编码发夹状siRNA寡聚核苷酸。将合成的序列互补的寡聚核苷酸退火形成双链,克隆至pSilencer 1.0-U6载体中,并转化DH5a菌株,氨苄抗性筛选阳性菌落,扩大培养后,提取质粒进行酶切鉴定,并进行测序分析。结果显示,酶切后得到预期长度的DNA片段;插入序列插入的位置与设计完全一致,无碱基缺失或突变。表明A型流感病毒NP基因小干涉RNA表达载体构建成功。 |
| 关键词: RNA干扰 流感病毒 NP基因 |
| DOI： |
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| Cloning of the recombinant plasmid targeting avian influenza virus gene by RNA interfering |
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| Abstract: |
| Here three pairs of DNA sequences which can encode short hairpin RNAs(shRNA) targeting at the NP gene of the avian influenza virus were designed and synthesized.The complement form was obtained by annealing and inserted into vector pSilencer1.0-U6.The recombinant plasmid was transformed into DH5a strains.The plasmid identified by restriction enzyme was used for sequence analysis.Results showed that the recombinant plasmids were successfully constructed,which provided a base for the further searching new therapy method and studying gene funtions of influenza A virus. |
| Key words: RNA interfering,influenza virus,NP gene |
