| 摘要: |
| 无菌采取犬血、肝素抗凝、分离出红细胞,制备不同浓度的红细胞悬液,接种24孔板中进行培养,在培养不同时间分别收集红细胞培养上清液,采用淋巴细胞增殖转化试验,检测其单独及协同不同丝裂原(PHA-P、ConA和LPS)对犬淋巴细胞增殖转化的影响,淋转结果用MTT法测定。结果显示,无论单独或是协同不同丝裂原(PHA-P、ConA和LPS),红细胞培养上清液对淋巴细胞均有明显的促增殖转化作用(P<0.05)；单独及协同丝裂原PHA-P、ConA和LPS刺激淋巴细胞时,红细胞浓度分别为1×108,1×109,5.0×107和1×108 mL-1,培养时间分别为56,72,56和72 h的红细胞培养上清效果最佳。表明,犬红细胞培养上清液对犬淋巴细胞的增殖转化有明显的促进作用。 |
| 关键词: 犬 红细胞培养上清液 淋巴细胞转化增殖试验 MTT法 |
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| 基金项目:陕西省自然科学基金项目（99SM01)；陕西省教育学院教师科研基金项目（06KJ023） |
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| Effects of canine erythrocyte cultural supernatants on promoting canine lymphocyte proliferation |
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| Abstract: |
| To understand the way of erythrocyte in regulation lymphocyte proliferation, different concentration canine erythrocytes were cultured and its supernatants were obtained in different cultural time. Then,these supernatants were added into canine lymphocyte cultural systems,and the lymphocyte proliferation was detected by MTT essay.The results show that canine erythrocyte supernatants can promote canine lymphocyte proliferation.For cultural supernatant of canine erythrocyte only and stimulating with different mitogens（PHA-P,ConA，LPS）,the best level combinations to activate canine lymphocytes proliferation are 1×108，1×108,5.0×107 and 1×108 mL-1 canine erythrocyte separately,corresponding with canine erythrocyte culture time 56，72，56 and 72 h.The results indicate that the effects of canine erythrocyte cultural supernatants on promoting canine lymphocyte prolifeeration is significant. |
| Key words: canine erythorcyte cultural supernatant of erythrocyte lymphocyte proliferation test MTT essay |
