| 摘要: |
| 应用免疫组织化学SP法检测γ-干扰素(Interferon-γ,IFN-γ)在不同生殖周期奶山羊卵巢中的表达情况,探讨IFN-γ的变化规律及其与生殖的关系。结果显示,在间情期三级卵泡颗粒细胞胞膜、发情期三级卵泡和成熟卵泡颗粒细胞、妊娠期卵泡颗粒细胞、妊娠7周粒黄体细胞、妊娠12,16和20周膜黄体细胞胞核中均有IFN-γ的表达;在三级卵泡和成熟卵泡的卵泡液中也有IFN-γ的表达;妊娠12,16和20周粒黄体细胞上IFN-γ表达量很低。IFN-γ可能在不同类型的细胞上发挥着不同的作用。 |
| 关键词: 山羊 IFN-γ 卵巢 颗粒细胞 黄体 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金项目(2001AA203081)；陕西省自然科学基金项目（2002C104） |
|
| Expression of interferon-γ in dairy goat ovary during different physiological periods |
|
|
| Abstract: |
| In this experiment,the method of supersensitive immunohistochemistry SP is used to detect the expression of the Interferon-γ(IFN-γ) during the different period of reproduction in the dairy goat ovary,and explore the variation regularity of IFN-γ and relationship between IFN-γ and reproduction. The results show that IFN-γ expression exists in cytomembrane of granulated cells in late tertiary follicle during dioestrus,granulated cells late tertiary follicle and mature follicle during oestrum,ovarian follicle during trimester of pregnancy, granulosa lutein cell in the 7th week of pregnancy,nucleus of theca lutein cell in the 12th,16th,20th week of pregnancy.The IFN-γ expression exists in the follicular fluid of late tertiary follicle and mature follicle,too.While,in granulosa lutein cell in the 12th,16th,20th week of pregnancy,the expression of the IFN-γ is few.It can be concluded that the IFN-γ exerts different roles in different types of cells. |
| Key words: goat IFN-γ ovary corpus luteum granulated cell |
