| 摘要: |
| 以加工番茄BO2和BO4无菌苗的子叶和下胚轴为外植体，以MS为基本培养基，研究了不同浓度的ZT，6-BA与不同浓度的IAA组合对外植体芽诱导的影响。结果表明，在不同浓度的ZT和IAA组合中，对2个番茄品种的子叶和下胚轴外植体芽诱导均以MS＋1.0 mg/LZT＋0.05mg/LIAA培养基为最好，BO2品种子叶和下胚轴的出芽率分别为32.7％和24.5％，BO4品种的分别为37.3％和28.2%；在不同浓度的6-BA和IAA组合中，诱导BO2外植体出芽率最高的培养基是MS＋2.0 mg/L 6-BA＋0.2 mg/LIAA，子叶和下胚轴的出芽率分别为27.3％和14.5％；诱导BO4品种子叶和下胚轴出芽率最高的培养基分别为MS＋1.0 mg/L6-BA＋0.2 mg/L IAA和MS＋2.0 mg/L6-BA＋0.2 mg/LIAA，子叶和下胚轴的出芽率均为28.2％；在MS＋0.1 mg/L IAA培养基上，加工番茄BO2再生芽1周左右就能长出根，形成完整的小植株。 |
| 关键词: 加工番茄 子叶 下胚轴 植株再生 |
| DOI： |
| 分类号: |
| 基金项目:西北农林科技大学优秀青年人才专项（08080214）；新疆建设兵团博士点基金 |
|
| Study on cotyledon and hypocotyl plant regeneration of processing tomato in vitro |
|
|
| Abstract: |
| Cotyledons and hypocotyls of BO2 and BO4 process tomatoes were used as explants to study the effects of different concentrations of ZT,6-BA combined with different concentrations of IAA on bud induction.The results showed that the best shoot induction medium for cotyledon and hypocotyls explants was MS+ZT 1.0 mg/L+IAA 0.05 mg/L in different combinations of ZT and IAA.The shoot induction ratios were 32.7% and 24.5% for BO2 cotyledon and hypocotyls explants and 37.3% and 28.2% for BO4 cotyledon and hypocotyls explants respectively.Among different combinations of 6-BA and IAA,the best medium for BO2 was MS+6-BA 2.0 mg/L+IAA 0.2 mg/L and the shoot induction ratio was 27.3% and 14.5% for cotyledon and hypocotyls explants respectively.The most suitable induction medium for cotyledon and hypocotyls explants of BO4 was MS+6-BA 1.0 mg/L+IAA 0.2 mg/L and MS+6-BA 2.0 mg/L+IAA 0.2 mg/L respectively and the shoot induction ratio was 28.2%.The root could be induced after the regenerated buds were planted in MS+IAA 0.1 mg/L one week later. |
| Key words: processing tomato cotyledons hypocotyls plant regeneration |
