| 摘要: |
| 优化了红曲霉菌5040产生红曲色素的发酵工艺条件，研究了红曲霉菌5040在液体深层发酵过程中的色价变化规律，以及红曲色素中红色素和黄色素的胞内外分布情况。结果表明：（1）红曲霉菌5040发酵的最佳工艺条件为摇床转速170 r/min，温度32 ℃，pH 5.4，接种量90 mL/L。③整个发酵过程分为3个阶段：0～32 h是菌体大量合成阶段，色素产量较低；32～128 h是色素生成期，色素生成稳定且产量高；128 h以后菌体生成和产色素能力下降。整个发酵期在0～128 h色素增长较为均匀。⑧胞外红色素含量高于胞内，胞内外黄色素的分布不呈规律性，黄色素总量高于红色素，整个发酵液色渊呈黄色。 |
| 关键词: 红曲霉菌 红曲色素 色价 红色素 黄色素 |
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| 基金项目:国家西部专项（2001BA901A19）；霍英东基金项目（81065）；国家“十五”科技攻关项目（2001BA501A15-2.3） |
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| Study on technical conditions and characteristics of pigments by Monascus anka 5040 |
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| Abstract: |
| The fermentation conditions of Monascus anka 5040 was optimized to produce monascus pigments;And the change regulation of colour values by Monascus anka 5040 in submerged culture and the distribution of red and yellow pigments in monascus pigments were studied.The results showed that:① The optimum fermentation conditions of Monascus anka 5040 were 170 r/min table rotational speed,32 ℃temperature,pH5.4 and 90 mL/L load.② There were three phases in the fermentation course:a lot of the Monascus anka grew in the 0-32 h of fermentation,and the pigments were produced stably and largely in the 32-128 h,the Monascus anka and the pigments were reduced after 128 h;the pigments were produced stably in the 0-128 h of fermentation.③ The content of red pigments inside cells were more than that outside cells.There was no regulation about the distribution of yellow pigments inside and outside cells,and the contents of yellow pigments were more than that of red ones,the liquid appeared yellow. |
| Key words: Monascus anka monascus pigments colour values red pigments yellow pigments |
