| 摘要: |
| 根据GenBank中cry2Ad基因序列设计1对特异性引物,以苏云金芽孢杆菌新菌株S249质粒DNA为模板,应用PCR扩增技术得到了1条大小约为2.0 kb的DNA片段（cry2Ad2）。通过引物步行法测定该片段长1919 bp,其中开放阅读框（ORF）长1902 bp,编码633个氨基酸;经DNASTAR软件分析,预测其蛋白分子量为70.72 ku,等电点pI=8.26.序列比较结果表明,该基因与cry2Ad类基因高度同源（同源性达99%）。该基因已在GenBank中登录,登录号为DQ219823,并被Btδ-内毒素基因国际命名委员会正式命名为cry2Ad2。 |
| 关键词: 苏云金芽孢杆菌 cry2Ad2；微生物杀虫剂 PCR；克隆 序列分析 |
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| 基金项目:国家“十五”攻关项目(2002BA516A04);西北农林科技大学研究生创新计划项目(05ych007) |
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| Cloning and characteristic analysis of cry2Ad2 gene from Novel Strain S249 of Bacillus thuringiensis |
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| Abstract: |
| cry2Ad2 gene was cloned by PCR procedure from Novel Strain S249 of Bacillus thuringiensis plasmid with the primers designed according to the cry2Ad sequence in the GenBank.Sequencing was done to the fragments by using primer walking method and its length was 1 919 bp.cry2Ad2 contained 1 902 bp ORF that predicted a protein of 633 amino acid with Mr=70.72 ku and pI=8.26.The amino acid sequence of cry2Ad2 gene showed 99% identity to other cry2Ad,and the sequence was registered in GenBank (Accession Number is DQ219823) which was designated cry2Ad2 gene as a novel gene by Bt delta-endotoxin nomenclature committee. |
| Key words: Bacillus thuringiensis cry2Ad2 microbial pesticide PCR cloning sequence analysis |
