| 摘要: |
| 以野生黄木香茎段为外植体，探讨了不同生长调节物质对其不定芽诱导、增殖和生根的影响，并建立了野生黄木香的高效再生体系。结果表明，利于野生黄木香分化的诱导培养基为MS+AgNO3 3.0 mg/L+6-BA 1.5 mg/L+NAA 0.5 mg/L+琼脂6.5 g/L+蔗糖30 g/L；以MS+AgNO3 3.0 mg/L+6-BA 1.5 mg/L+NAA 0.05 mg/L+琼脂6.5 g/L+蔗糖30 g/L为增殖培养基时的增殖效果最好，增殖倍数为5.5；黄木香高效的再生体系为1/2 MS+AgNO3 4.5 mg/L+IBA 0.2 mg/L+质量分数0.2%活性炭+琼脂4.0 g/L+蔗糖20 g/L，其生根率可达95 %。 |
| 关键词: 野生黄木香 不定芽 快速繁殖 植物生长调节剂 |
| DOI： |
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| 基金项目:河南省南阳市2004年科技攻关项目（200404016）；南阳师范学院2003年应用与科技开发项目（NYTC200318） |
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| Culturing of wild Rosa banksiae f.lutescecs Vots.in vitro and plant regeneration system |
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| Abstract: |
| By taking wild Rosa banksiae f.lutescecs Vots.as explants,the influence of different growth regulating substances on adventitious buds's induction,multiplication and rooting was studied,and the regeneration system of high efficiency on Rosa banksiae f.lutescecs Vots.was established.The results suggested that the best medium for induction was MS+AgNO3 3.0 mg/L+6-BA 1.5 mg/L+NAA 0.5 mg/L+agar 6.5 g/L+sucrose 30 g/L;the best medium for the multiplication was MS+AgNO3 3.0 mg/L+6-BA 1.5 mg/L+NAA 0.05 mg/L+agar 6.5 g/L+sucrose 30 g/L,and the multiplication multiple was 5.5；the regeneration system of high efficiency on Rosa banksiae f.lutescecs Vots.was 1/2 MS+AgNO3 4.5 mg/L+IBA 0.2 mg/L+0.2% activated carbon+agar 4.0 g/L+sucrose 20 g/L，and the rooting rate was 95%. |
| Key words: wild Rosa banksiae f.lutescecs Vots. adventitious buds rapid propagation plant growth regulator |
