| 摘要: |
| 设置养分贮存损失状态不同的4类培养液，分别添加一定浓度梯度的L 谷氨酰胺(L-Glutamine,L-Gln)培养大鼠前体脂肪细胞。通过形态学观察、MTT比色、油红O染色提取法,比较各组细胞形态及增殖与分化的效果。结果表明，添加0 mmol/L L-Gln的新鲜培养液和配制后4 ℃冷藏20 d，再添加0.685 mmol/L L-Gln的培养液，均较利于前体脂肪细胞的增殖与分化；配制后4 ℃冷藏90 d的培养液，对前体脂肪细胞培养效果较差，添加L-Gln也不能使培养效果得到改善。另外，启封后的M199和胎牛血清（Fetal Calf Serum，FCS）分别冷藏不宜超过90 d。说明及时满足培养液中L-Gln的需求量，可使前体脂肪细胞达到较好的增殖与分化状态。 |
| 关键词: 培养液 L-Gln 大鼠 前体脂肪细胞 增殖与分化 |
| DOI： |
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| 基金项目:国家自然科学基金项目（30471239） |
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| Effect of L-Gln on proliferation and differentiation of rat preadipocytes |
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| Abstract: |
| L-Gln was added to the 4 culture media of different nutritions for rat preadipocytes.The proliferation and differentiation of preadipocyte were determined by MTT spectrophotometry and Oil Red O staining respectively.The results show that the addition of 0 mmol/L L-Gln in fresh culture media and 0.685 mmol/L L-Gln in the culture media which had been stored at 4 ℃ for 20 d benefited proliforation and differentiation of the preadipocytes.When culture media was stored at 4 ℃ for 90 d,it could not make the preadipocytes develop soundly,even when L-Gln was added.Once more,M199 and FCS (fetal calf serum) could not be stored at 4 ℃ beyond 90 d if they were opened.The above shows that suitable supplement of L-Gln could make preadipocytes approach better states of proliferation and differention. |
| Key words: culture media L-Gln rat preadipocyte proliferation and differentiation |
