| 摘要: |
| 从猪胃窦和十二指肠提取总RNA，采用美国Ambion RNA公司生产的Quantum RNA classic 18S为内标，建立了半定量RT-PCR方法，并对胃窦和十二指肠胃泌素mRNA进行了定量检测、测序和比较分析。结果表明，扩增的猪胃窦和十二指肠胃泌素核苷酸序列与猪前胃泌素（propregastrin）核苷酸的同源性达到99％。将其与已发表的人、牛、大鼠和小鼠等物种相应序列的比较结果表明，无论是核苷酸水平，还是氨基酸水平，都显示出了较高的同源性（80％～99％）。另外，建立的RT-PCR方法灵敏度高，操作快捷方便，可用于定量检测少量的组织样品中低丰度表达的胃泌素mRNA。 |
| 关键词: RT-PCR 胃泌素 同源性分析 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金项目（30070565） |
|
| Establishment of gastrin relative quantification RT-PCR and analysis of its homology |
|
|
| Abstract: |
| RNA was extracted from stomach and duodenum in pigs.Relative RTPCR using Quantum RNATM 18 S internal Standards for the quantitation of Gastrin mRNA was established for examining in present study.The 99% of nuclei length of the product from RT-PCR was identical with porcine messenger RNA for preprogastrin.Comparision of this sequence with the sequences of human,bovine,mouse and rat,both nucleotide composition and codon usage amino acid comparision were more homological (80%-99%).The result indicated that RT-PCR was much more sensitive and reliable in detecting lowly expressed mRNAs in small tissues. |
| Key words: RT-PCR gastrin homological analysis |
