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| 摘要: |
| 通过对影响RAPD标记稳定性的引物浓度梯度、退火温度梯度、dNTP浓度梯度、Taq酶浓度梯度等外部条件的优化研究，确定了RAPD反应适宜的引物浓度为0.1～0.2 pmol/μL,退火温度为48～52 ℃,dNTP浓度为0.3～0.15 mmol/L及Taq酶浓度为0.3 U/μL，提高了该反应体系中RAPD的稳定性。 |
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| DOI： |
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| 基金项目:农业部948项目(991055)；蔬菜遗传与生理重点实验室资助项目 |
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| Optimizing conditions affecting the stability of RAPD |
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| Abstract: |
| Factors that affect the stability of RAPD were optimized such as primer concentration grads,annealing temperature grads,dNTP concentration grads and taq concentration grads.The proper concentration of primer was 0.1-0.2 pmol/μL,the proper annealing temperature 48-52 ℃,the proper concentration of dNTP 0.3-0.15 mmol/L,the proper Taq concentration 0.3 U/μL.and the stability of RAPD was enhanced in this system. |
| Key words: RAPD primer concentration grads annealing template concentration grads dNTP concentration grads Taq concentration grads |
