| 摘要: |
| 通过PCR扩增出2.2 kh的山羊β-乳球蛋白基因5′端的调控序列，包括第一外显子和第一内含子。测序结果表明，该序列与GenBank中登录序列相比，同源性为99.5％。用其替代表达载体pEGFP-c1上原来的启动子CMV，指导报告基因绿色荧光蛋白(GFP)在山羊乳腺细胞中的瞬时表达，结果发现该调控序列可以有效指导报告基因的表达，从而初步验证了所克隆的山羊乳球蛋白(BLG)基因表达调控序列的有效性。表明克隆的调控序列可用于乳腺生物反应器的研究。 |
| 关键词: 山羊 β-乳球蛋白基因 GFP基因 |
| DOI： |
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| 基金项目:国家“863”高技术研究与发展计划项目(2001AA213081) |
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| Cloning of 5 flanking sequence of goat BLG and regulating the expression of GFP in cell |
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| Abstract: |
| 5′flanking region of goat BLG was amplified from goat genomic DNA according to the published whole sequence of goat BLG and cloned to pMD-18t vector correspondently.By sequencing,the amplified sequence was the same as 99% as publication,with which replaced previous promoter CMV cloned in pEGFP-c1 expressional vector and transfected the cultured Mammary cell in vivo.Through observation under UV microscope,it showed that GFP had been successfully expressed in Mammary Gland cell.Then,the cloned sequence can be served for the study of mammary gland expression construct. |
| Key words: goat β-lactoglobulin gene green fluorescent protein gene |
