| 摘要: |
| 将胸腺细胞分为正常对照组、H2O2模型组、0.1 g/L Vc组、0.5 g/L PCF组、0.25 g/L PCF组和0.125 g/L PCF组6组，通过不同药物或不同浓度处理后，采用生物化学方法分别检测细胞裂解液中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、丙二醛（MDA）、总抗氧化能力（T-AOC）和活性氧（ROS）的含量，采用流式细胞仪测定各组细胞胞浆中的Ca2+含量和线粒体膜电位(ΔΨm)的变化。结果显示，扇贝多肽(PCF)能提高细胞中SOD,GSH-Px的含量，增强细胞的T-AOC，降低细胞中MDA和ROS的含量，并能引起胞浆中Ca2+含量的下降和ΔΨm的提升。表明PCF具有抑制H2O2对胸腺细胞氧化损伤的作用。 |
| 关键词: 栉孔扇贝 胸腺细胞 过氧化氢 抗氧化剂 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金项目(39970638) |
|
| Protective effects of polypeptide from Chlamys farreri on thymocytes damaged by hydrogen peroxide |
|
|
| Abstract: |
| To study the protective effects of polypeptide from Chlamys farreri (PCF) on thymocytes damaged by H2O2 and antioxidative mechanisms of PCF,we made a thymocytes damaged-H2O2 model.In this study,thymocytes were divided into six groups including control group,model group,0.1 g/L Vc group,0.5 g/L PCF group,0.25 g/L PCF group and 0.125 g/L PCF group.Thymocytes in different groups were exposed to PCF at various concentrations and 12.5 μmol/L H2O2.Levels of superoxide dismutase (SOD),glutathione peroxidases (GSH-Px),Total Antioxidative Capacity (T-AOC),malondialdehyde (MDA) and reactive oxygen species (ROS) in cell lysates were estimated by biochemical methods.Cytosolic Ca2+ levels and mitochondria membrane potential (ΔΨm) were determined by flow cytometry.Results showed PCF could enhance the activities of SOD and GSH Px,promote antioxidative capacity and reduce the amount of MDA and ROS.Moreover,PCF caused an elevation of ΔΨm and a decrease of cytosolic Ca2+.It is concluded that PCF can inhibit the damaging effect of H2O2. |
| Key words: Chlamys farreri thymocyte hydrogen peroxide antioxidant |
