| 摘要: |
| 对野油菜黄单胞菌的α-淀粉酶进行了定位,确定该酶为胞外酶,且只有一型α-淀粉酶.采用切胶纯化得到纯酶,SDS-PAGE测得其相对分子质量为52 ku.研究显示,该酶的最适作用温度为50 ℃,最适pH值为6.2,通过酶的稳定性研究发现该酶热稳定性较差,而pH值在4.86～10.47具有较好的稳定性. |
| 关键词: 野油菜黄单胞菌 α-淀粉酶 酶学性质 |
| DOI： |
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| 基金项目:河南省科技厅重点科技攻关项目(0223013500) |
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| Study on properties of α-armylase from Xanthomonas campestris pv.campestris 8004 |
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| Abstract: |
| The α-amylase derived from Xanthomonas campestris pv.campestris 8004 was confirmed as extracellular enzyme by two totally different methods and there was only one type α-amylase existing.The α-amylase was purified by PAGE,and SDS-PAGE indicated its molecular weight was 52 ku.The optimum temperature and pH of this enzyme was 50 ℃ and pH 6.2.Stability tests showed that this α-amylase was unstable at higher temperature,yet it was quite stable in wide pH range of 4.86-10.47. |
| Key words: Xanthomonas campestris pv.compestris α-amylase enzyme property |
