| 摘要: |
| 根据GenBank中已发表的猪传染性胃肠炎病毒（TGEV）基因序列，利用Primer 5.0程序软件，设计并合成了M基因的2对引物，以mRNA为模板，通过RT-PCR,RT-nested PCR方法，成功扩增出长度为1 328 bp和1 037 bp的TGEV目的片段，但未扩增出猪传染性腹泻病毒（PEDV）片段。在优化RT-PCR反应条件的基础上，建立了快速检测TGEV的诊断方法。结果表明,RT-nested PCR方法可用于检测猪传染性胃肠炎病毒，而且此方法简单省时、灵敏性高，可以作为检测RNA病毒的一种分子生物学方法。 |
| 关键词: 猪传染性胃肠炎病毒 RT-PCR RT-nested PCR M基因 |
| DOI： |
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| 基金项目:国家“863”高技术资助项目(2001AA213081) |
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| Study on detecting transmissible gastroenteritis virus by reverse transcription-nested polymerase chain reaction |
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| Abstract: |
| Two pairs of oligonucleotide primers appropriate for PCR amplication were selected based on the gene encoding of the M protein of TGEV.The amplified products of TGEV were 1 328 bp,1 037 bp respectively,but as to PEDV nothing was amplified.By a reverse transcription-nested polymerase chain reaction (RT-nested PCR) assay,a quick detection of TGEV after optimization of the reaction conditions was developed.The results showed that RT-PCR assay can be used to detect the virus of RNA. |
| Key words: TGEV PT-PCR PT-nested PCR M gene |
