| 摘要: |
| 根据GeneBank(U37518)中TRAIL(肿瘤坏死因子相关的凋亡诱导配体)的cDNA序列，设计扩增引物。采用RT-PCR从人胎盘中扩增出TRAIL基因的全长cDNA。产物纯化后连至pGEM-T Easy载体，转化大肠杆菌DH5α。筛选阳性克隆菌，酶切鉴定并进行序列测定。结果表明，本试验成功地克隆了TRAIL基因的1 039bp全长cDNA。 |
| 关键词: 肿瘤坏死因子相关的凋亡诱导配体（TRAIL） RT-PCR 序列测定 人胎盘 |
| DOI： |
| 分类号: |
| 基金项目:国家“863”高技术资助项目(2001AA213081) |
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| Cloning and sequencing of cDNA of TRAIL from human placenta |
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| Abstract: |
| The financial risk of universities and colleges is produced with the obtainment of legal person position and expansion of independence as well as the change of educational structure and scales. The three main risks are unbalanced risk of financial situation, debt risk and risk caused by industries run by universities and colleges. The causes of each risk are different, and the precautions mechanism should be established respectively. |
| Key words: TNF-related apoptosis-inducing ligand (TRAIL),RT-PCR,sequencing,human placenta |
